BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4620

Title: Human prion protein variant R220K

Deposition date: 2000-05-11 Original release date: 2000-12-06

Authors: Calzonai, L.; Lysek, D.; Guntert, P.; Von Schroetter, C.; Zahn, R.; Riek, R.; Wuthrich, K.

Citation: Calzonai, L.; Lysek, D.; Guntert, P.; Von Schroetter, C.; Zahn, R.; Riek, R.; Wuthrich, K.. "NMR structures of three single-residue variants of the human prion protein"  Proc. Natl. Acad. Sci. U. S. A. 97, 8340-8345 (2000).

Assembly members:
prion protein, polymer, 112 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
prion protein: GSVVGGLGGYMLGSAMSRPI IHFGSDYEDRYYRENMHRYP NQVYYRPMDEYSNQNNFVHD CVNITIKQHTVTTTTKGENF TETDVKMMERVVEQMCITQY EKESQAYYQRGS

Data sets:
Data typeCount
13C chemical shifts355
15N chemical shifts125
1H chemical shifts768

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1prion protein1

Entities:

Entity 1, prion protein 112 residues - Formula weight is not available

1   GLYSERVALVALGLYGLYLEUGLYGLYTYR
2   METLEUGLYSERALAMETSERARGPROILE
3   ILEHISPHEGLYSERASPTYRGLUASPARG
4   TYRTYRARGGLUASNMETHISARGTYRPRO
5   ASNGLNVALTYRTYRARGPROMETASPGLU
6   TYRSERASNGLNASNASNPHEVALHISASP
7   CYSVALASNILETHRILELYSGLNHISTHR
8   VALTHRTHRTHRTHRLYSGLYGLUASNPHE
9   THRGLUTHRASPVALLYSMETMETGLUARG
10   VALVALGLUGLNMETCYSILETHRGLNTYR
11   GLULYSGLUSERGLNALATYRTYRGLNARG
12   GLYSER

Samples:

sample_1: prion protein, [U-13C; U-15N], 1.0 mM; sodium acetate 50 mM

sample_cond_1: ionic strength: 50 mM; pH: 4.5; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
HNCAsample_1not availablesample_cond_1
HN(CO)CAsample_1not availablesample_cond_1
HCCH-TOCSYsample_1not availablesample_cond_1
15N-resolved (1H,1H)NOESYsample_1not availablesample_cond_1
13C-resolved (1H,1H)NOESYsample_1not availablesample_cond_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 750 MHz

Related Database Links:

BMRB 4379 4402 4434 4641 4736 5713
PDB
DBJ BAA00011 BAG32276 BAG35206 BAG52189 BAG56832
EMBL CAA58442 CAB75503 CAM27320
GenBank AAA19664 AAA60182 AAA68633 AAB59442 AAB59443
REF NP_000302 NP_001073590 NP_001073591 NP_001073592 NP_898902
SWISS-PROT P04156 P40252

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts