BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4669

Title: Structural Analysis of the Immunodominant Antigenic Region of the Hepatitis C Virus Capsid Protein by NMR

Deposition date: 2000-02-25 Original release date: 2002-04-01

Authors: Ladaviere, L.; Deleage, G.; Montserret, R.; Dalbon, P.; Jolivet, M.; Penin, F.

Citation: Ladaviere, L.; Deleage, G.; Montserret, R.; Dalbon, P.; Jolivet, M.; Penin, F.. "Structural Analysis of the Immunodominant Antigenic Region of the Hepatitis C Virus Capsid Protein by NMR"  . ., .-..

Assembly members:
HEPATITIS C VIRUS CAPSID PROTEIN, polymer, 44 residues, 4900 Da.

Natural source:   Common Name: hepatitis C virus   Taxonomy ID: 11103   Superkingdom: viruses   Kingdom: not available   Genus/species: hepatitis C virus hepatitis C virus

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
HEPATITIS C VIRUS CAPSID PROTEIN: STNPKPQRKTKRNTNRRPQD VKFPGGGQIVGGVYLLPRRG PRLG

Data sets:
Data typeCount
1H chemical shifts317

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HCV cap(2,45)1

Entities:

Entity 1, HCV cap(2,45) 44 residues - 4900 Da.

1   SERTHRASNPROLYSPROGLNARGLYSTHR
2   LYSARGASNTHRASNARGARGPROGLNASP
3   VALLYSPHEPROGLYGLYGLYGLNILEVAL
4   GLYGLYVALTYRLEULEUPROARGARGGLY
5   PROARGLEUGLY

Samples:

sample_1: HEPATITIS C VIRUS CAPSID PROTEIN mM; Trifluoroethanol, [U-2H], 40%; Sodium phosphate 0.01 M; NaCl 0.1 M

sample_cond_1: pH: 5.9; temperature: 293 K; ionic strength: 0.1 M

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablesample_cond_1
DQF-COSYsample_1not availablesample_cond_1
2D ROESYsample_1not availablesample_cond_1
2D TOCSYsample_1not availablesample_cond_1

Software:

VNMR v5.1 - DATA COLLECTION, DATA ANALYSIS

X-PLOR v3.1 - REFINEMENT, STRUCTURE SOLUTION

NMR spectrometers:

  • Varian UnityPlus 500 MHz

Related Database Links:

BMRB 15767 15768
PDB
DBJ BAA00452 BAA00705 BAA00706 BAA00792 BAA00968
EMBL CAA03854 CAA43788 CAA43790 CAA43791 CAA43792
GB AAA16300 AAA16311 AAA20153 AAA20154 AAA21028
PIR JQ1926
PRF 2012309A 2116349AA 2116349AB 2116349AC 2116349AD
REF NP_671491 NP_751919 YP_001469630 YP_001469632 YP_001469633
SP O39928 O39929 O91936 O92972 P26660