BMRB Entry 4733
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4733
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Title: HMG-D complexed to a bulge DNA: an NMR study
Deposition date: 2000-05-03 Original release date: 2000-05-11
Authors: Cerdan, Rachel; Payet, Dominique; Yang, Ji-Chun; Travers, Andrew; Neuhaus, David
Citation: Cerdan, Rachel; Payet, Dominique; Yang, Ji-Chun; Travers, Andrew; Neuhaus, David. "HMG-D complexed to a bulge DNA: an NMR study" Protein Sci. ., .-. (2000).
Assembly members:
bulge DNA 12/14mer, polymer, 14 residues, Formula weight is not available
bulge DNA 12/14mer, polymer, 12 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
bulge DNA 12/14mer: CGATATTAAGAGCC
bulge DNA 12/14mer: GGCTCAATATCG
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 203 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | bulge DNA free 12/14mer strand 1 | 1 |
| 2 | bulge DNA free 12/14mer strand 2 | 2 |
Entities:
Entity 1, bulge DNA free 12/14mer strand 1 14 residues - Formula weight is not available
| 1 | DC | DG | DA | DT | DA | DT | DT | DA | DA | DG | ||||
| 2 | DA | DG | DC | DC |
Entity 2, bulge DNA free 12/14mer strand 2 12 residues - Formula weight is not available
| 1 | DG | DG | DC | DT | DC | DA | DA | DT | DA | DT | ||||
| 2 | DC | DG |
Samples:
sample_1: bulge DNA 12/14mer 1 mM; bulge DNA 12/14mer 1 mM
cond_1: ionic strength: 0.005 M; pH: 6.0; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | sample_1 | not available | cond_1 |
| 2D 1H-15N HSQC | sample_1 | not available | cond_1 |
| 3D HNCA | sample_1 | not available | cond_1 |
| 3D CBCA(CO)NH | sample_1 | not available | cond_1 |
Software:
Xwin-NMR -
FELIX -
NMR spectrometers:
- Bruker DMX 600 MHz
- Bruker Avance 800 MHz