BMRB Entry 4750
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4750
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Title: NMR structure of the most conserved RNA motif in Srp RNA
Deposition date: 2000-06-02 Original release date: 2000-07-06
Authors: Schmitz, U.; James, T.; Lukavsky, P.; Walter, P.
Citation: Schmitz, U.; James, T.; Lukavsky, P.; Walter, P.. "Structure of the Most Conserved internal loop in Srp RNA" Nat. Struct. Biol. 6, 634-638 (1999).
Assembly members:
SRP DOMAIN IV, polymer, 28 residues, 8000 Da.
Natural source: Common Name: Escherichia coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: enzymatic semisynthesis Host organism: Escherichia coli
Entity Sequences (FASTA):
SRP DOMAIN IV: GGCGUCAGGUCCGGAAGGAA
GCAGCGCC
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 214 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SRP DOMAIN IV | 1 |
Entities:
Entity 1, SRP DOMAIN IV 28 residues - 8000 Da.
| 1 | G | G | C | G | U | C | A | G | G | U | ||||
| 2 | C | C | G | G | A | A | G | G | A | A | ||||
| 3 | G | C | A | G | C | G | C | C |
Samples:
sample_1: SRP DOMAIN IV 0.25 mM
sample_cond_1: pH: 6.5; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NOESY, | not available | not available | not available |
| COSY, | not available | not available | not available |
| SSNOESY, | not available | not available | not available |
| TOCSY | not available | not available | not available |
Software:
AMBER v5.0 - REFINEMENT
SPARKY - STRUCTURE CALCULATION
NMR spectrometers:
- Varian Unity Plus 600 MHz