BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4787

Title: Assignment of 1H, 13C and 15N resonances of domain III of the ectodomain of apical membrane antigen 1 from Plasmodium falciparum

Deposition date: 2000-07-19 Original release date: 2001-02-14

Authors: Nair, Margie; Hodder, Anthony; Hinds, Mark; Anders, Robin; Norton, Raymond

Citation: Nair, Margie; Hodder, Anthony; Hinds, Mark; Anders, Robin; Norton, Raymond. "Letter to the Editor: Assignment of 1H, 13C and 15N resonances of domain III of the ectodomain of apical membrane antigen 1 from Plasmodium falciparum"  J. Biomol. NMR 19, 85-86 (2001).

Assembly members:
Apical membrane antigen 1, polymer, 122 residues, 14330 Da.

Natural source:   Common Name: malaria parasite   Taxonomy ID: 5833   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Plasmodium falciparum

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Apical membrane antigen 1: MRGSHHHHHHGSEVENNFPC SLYKDEIMKEIERESKRIKL NDNDDEGNKKIIAPRIFISD DKDSLKCPCDPEMVSNSTCR FFVCKCVERRAEVTSNNEVV VKEEYKDEYADIPEHKPTYD KM

Data sets:
Data typeCount
1H chemical shifts670
13C chemical shifts358
15N chemical shifts96

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Pf AMA 1 domain III1

Entities:

Entity 1, Pf AMA 1 domain III 122 residues - 14330 Da.

1   METARGGLYSERHISHISHISHISHISHIS
2   GLYSERGLUVALGLUASNASNPHEPROCYS
3   SERLEUTYRLYSASPGLUILEMETLYSGLU
4   ILEGLUARGGLUSERLYSARGILELYSLEU
5   ASNASPASNASPASPGLUGLYASNLYSLYS
6   ILEILEALAPROARGILEPHEILESERASP
7   ASPLYSASPSERLEULYSCYSPROCYSASP
8   PROGLUMETVALSERASNSERTHRCYSARG
9   PHEPHEVALCYSLYSCYSVALGLUARGARG
10   ALAGLUVALTHRSERASNASNGLUVALVAL
11   VALLYSGLUGLUTYRLYSASPGLUTYRALA
12   ASPILEPROGLUHISLYSPROTHRTYRASP
13   LYSMET

Samples:

15N_sample: Apical membrane antigen 1, [U-90% 15N], 0.5 – 1 mM

13C_sample: Apical membrane antigen 1, [U-95% 13C[, 0.5 – 1 mM

sample_conditions_set_1: pH: 3.4; temperature: 303 K

sample_conditions_set_2: pH: 3.4; temperature: 283 K

sample_conditions_set_3: pH: 2.8; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-1H-15N NOESYnot availablenot availablenot available
3D 1H-1H-13C NOESYnot availablenot availablenot available
3D 1H-1H-15N TOCSYnot availablenot availablenot available
3D HNCAnot availablenot availablenot available
3D HNCOnot availablenot availablenot available
3D CBCA(CO)NHnot availablenot availablenot available
3D CCCONHnot availablenot availablenot available
3D 1H-13C-1H HCCH-TOCSYnot availablenot availablenot available
3D HNHAnot availablenot availablenot available

Software:

UXNMR v1.3 - Data processing

XEASY v1.3.13 - Peak assignment and analysis

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker AMX 500 MHz

Related Database Links:

PDB
DBJ BAM85373 BAM85381 BAM85383 BAM85388 BAM85400
EMBL CAB97182 CAB97190 CAB97197 CAC34760 CAC34761
GB AAA29476 AAB36701 AAC47104 AAC47105 AAG50119
REF XP_001348015

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts