BMRB Entry 4814
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4814
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Title: Complete 1H, 15N, and 13C assignments of an exchangeable apolipoprotein, Locusta migratoria apolipophorin III
Deposition date: 2000-08-24 Original release date: 2001-02-14
Authors: Fan, Daping; Reese, Leslie; Ren, Xuefeng; Weers, Paul; Ryan, Robert; Wang, Jianjun
Citation: Fan, Daping; Reese, Leslie; Ren, Xuefeng; Weers, Paul; Ryan, Robert; Wang, Jianjun. "Letter to the Editor: Complete 1H, 15N, and 13C assignments of an exchangeable apolipoprotein, Locusta migratoria apolipophorin III" J. Biomol. NMR 19, 83-84 (2001).
Assembly members:
apolipoprotein, polymer, 164 residues, 17501.92 Da.
Natural source: Common Name: not available Taxonomy ID: 7004 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Locusta migratoria
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
apolipoprotein: RPDAAGHVNIAEAVQQLNHT
IVNAAHELHETLGLPTPDEA
LNLLTEQANAFKTKIAEVTT
SLKQEAEKHQGSVAEQLNAF
ARNLNNSIHDAATSLNLQDQ
LNSLQSALTNVGHQWQDIAT
KTQASAQEAWAPVQSALQEA
AEKTKEAAANLQNSIQSAVQ
KPAN
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 159 |
| 1H chemical shifts | 1098 |
| 13C chemical shifts | 324 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | exchangeable apolipoprotein | 1 |
Entities:
Entity 1, exchangeable apolipoprotein 164 residues - 17501.92 Da.
| 1 | ARG | PRO | ASP | ALA | ALA | GLY | HIS | VAL | ASN | ILE | ||||
| 2 | ALA | GLU | ALA | VAL | GLN | GLN | LEU | ASN | HIS | THR | ||||
| 3 | ILE | VAL | ASN | ALA | ALA | HIS | GLU | LEU | HIS | GLU | ||||
| 4 | THR | LEU | GLY | LEU | PRO | THR | PRO | ASP | GLU | ALA | ||||
| 5 | LEU | ASN | LEU | LEU | THR | GLU | GLN | ALA | ASN | ALA | ||||
| 6 | PHE | LYS | THR | LYS | ILE | ALA | GLU | VAL | THR | THR | ||||
| 7 | SER | LEU | LYS | GLN | GLU | ALA | GLU | LYS | HIS | GLN | ||||
| 8 | GLY | SER | VAL | ALA | GLU | GLN | LEU | ASN | ALA | PHE | ||||
| 9 | ALA | ARG | ASN | LEU | ASN | ASN | SER | ILE | HIS | ASP | ||||
| 10 | ALA | ALA | THR | SER | LEU | ASN | LEU | GLN | ASP | GLN | ||||
| 11 | LEU | ASN | SER | LEU | GLN | SER | ALA | LEU | THR | ASN | ||||
| 12 | VAL | GLY | HIS | GLN | TRP | GLN | ASP | ILE | ALA | THR | ||||
| 13 | LYS | THR | GLN | ALA | SER | ALA | GLN | GLU | ALA | TRP | ||||
| 14 | ALA | PRO | VAL | GLN | SER | ALA | LEU | GLN | GLU | ALA | ||||
| 15 | ALA | GLU | LYS | THR | LYS | GLU | ALA | ALA | ALA | ASN | ||||
| 16 | LEU | GLN | ASN | SER | ILE | GLN | SER | ALA | VAL | GLN | ||||
| 17 | LYS | PRO | ALA | ASN |
Samples:
sample_1: apolipoprotein, [U-99% 13C; U-99% 15N], 0.6 – 1.0 mM
Normal-Cond: pH: 6.4; temperature: 303 K; ionic strength: 0.25 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| A 5 mm triple-resonance probe with z-gradient. The above pulse sequences are from Dr. | sample_1 | not available | Normal-Cond |
| Lewis Kay | not available | not available | not available |
| not available | not available | not available | not available |
Software:
NMRPIPE v1.7 - Spectral processing
PIPP v4.2.0 - Spectral assignment
NMR spectrometers:
- Varian INOVA 500 MHz
Download simulated HSQC data in one of the following formats:
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