BMRB Entry 4835
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4835
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Title: NMR Structure of the palindromic DNA decamer D(GCGTTAACGC)2
Deposition date: 2000-09-19 Original release date: 2000-12-07
Authors: Smith, J.; Tsui, V.; Chazin, W.; Case, D.
Citation: Smith, Jarrod. "DNA structure by 1H NMR methods: Studies of d(GCGTTAACGC)2" Thesis The Scripps Research Institute (1999).
Assembly members:
5'-d(*GP*CP*GP*TP*TP*AP*AP*CP*GP*C)-3', polymer, 10 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: .
Entity Sequences (FASTA):
5'-d(*GP*CP*GP*TP*TP*AP*AP*CP*GP*C)-3': GCGTTAACGC
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 82 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | GCG10 strand one | 1 |
| 2 | GCG10 strand two | 1 |
Entities:
Entity 1, GCG10 strand one 10 residues - Formula weight is not available
| 1 | DG | DC | DG | DT | DT | DA | DA | DC | DG | DC |
Samples:
sample_1: 5'-d(*GP*CP*GP*TP*TP*AP*AP*CP*GP*C)-3' 2 mM; Na3PO4 20 mM; NaCl 50 mM; NaN3 0.1%; D2O 99.9%
sample_2: 5'-d(*GP*CP*GP*TP*TP*AP*AP*CP*GP*C)-3' 2 mM; Na3PO4 20 mM; NaCl 50 mM; NaN3 0.1%; D2O 5%; H2O 95%
sample_cond_1: ionic strength: 70 mM; pH: 7; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| Double-Quantum | not available | not available | sample_cond_1 |
| DQF-COSY | not available | not available | sample_cond_1 |
| E-COSY | not available | not available | sample_cond_1 |
| 2D NOESY | not available | not available | sample_cond_1 |
Software:
FELIX v95 - processing
NAB v2.1 - structure calculation
AMBER v5.0 - refinement, iterative matrix relaxation
NMR spectrometers:
- Bruker AMX 500 MHz