BMRB Entry 4894

Name: RNA recognition by a staufen double-stranded RNA-binding domain
Published: 2000-12-06

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4894

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: RNA recognition by a staufen double-stranded RNA-binding domain

Deposition date: 2000-11-07 Original release date: 2000-12-06

Authors: Ramos, A.; Grunert, S.; Bycroft, M.; St Johnston, D.; Varani, G.

Citation: Ramos, A.; Grunert, S.; Adams, J.; Micklem, D.; Proctor, M.; Freund, S.; Bycroft, M.; St Johnston, D.; Varani, G.. "RNA recognition by a staufen double-stranded RNA-binding domain" EMBO J. 19, 997-1009 (2000).

Assembly members:
STAUFEN DOUBLE-STRANDED RNA BINDING DOMAIN, polymer, 90 residues, Formula weight is not available
RNA hairpin, polymer, 30 residues, Formula weight is not available

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
STAUFEN DOUBLE-STRANDED RNA BINDING DOMAIN: GSMDEGDKKSPISQVHEIGI KRNMTVHFKVLREEGPAHMK NFITACIVGSIVTEGEGNGK KVSKKRAAEKMLVELQKLPP LTPTKEFIVD
RNA hairpin: GGACAGCUGUCCCUUCGGGG ACAGCUGUCC

Data sets:

assigned_chemical_shifts
- chemical_shifts_set1
- chemical_shifts_set2

Data type	Count
13C chemical shifts	345
15N chemical shifts	93
1H chemical shifts	781
31P chemical shifts	4

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Entity Assembly ID	Entity Name	Entity ID
1	dsRBD3	1
2	RNA2	2

1	GLY	SER	MET	ASP	GLU	GLY	ASP	LYS	LYS	SER
2	PRO	ILE	SER	GLN	VAL	HIS	GLU	ILE	GLY	ILE
3	LYS	ARG	ASN	MET	THR	VAL	HIS	PHE	LYS	VAL
4	LEU	ARG	GLU	GLU	GLY	PRO	ALA	HIS	MET	LYS
5	ASN	PHE	ILE	THR	ALA	CYS	ILE	VAL	GLY	SER
6	ILE	VAL	THR	GLU	GLY	GLU	GLY	ASN	GLY	LYS
7	LYS	VAL	SER	LYS	LYS	ARG	ALA	ALA	GLU	LYS
8	MET	LEU	VAL	GLU	LEU	GLN	LYS	LEU	PRO	PRO
9	LEU	THR	PRO	THR	LYS	GLU	PHE	ILE	VAL	ASP

Biological Magnetic Resonance Data Bank

BMRB Entry 4894

Title: RNA recognition by a staufen double-stranded RNA-binding domain

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	GLY	SER	MET	ASP	GLU	GLY	ASP	LYS	LYS	SER
2	PRO	ILE	SER	GLN	VAL	HIS	GLU	ILE	GLY	ILE
3	LYS	ARG	ASN	MET	THR	VAL	HIS	PHE	LYS	VAL
4	LEU	ARG	GLU	GLU	GLY	PRO	ALA	HIS	MET	LYS
5	ASN	PHE	ILE	THR	ALA	CYS	ILE	VAL	GLY	SER
6	ILE	VAL	THR	GLU	GLY	GLU	GLY	ASN	GLY	LYS
7	LYS	VAL	SER	LYS	LYS	ARG	ALA	ALA	GLU	LYS
8	MET	LEU	VAL	GLU	LEU	GLN	LYS	LEU	PRO	PRO
9	LEU	THR	PRO	THR	LYS	GLU	PHE	ILE	VAL	ASP

1	GLY	SER	MET	ASP	GLU	GLY	ASP	LYS	LYS	SER
2	PRO	ILE	SER	GLN	VAL	HIS	GLU	ILE	GLY	ILE
3	LYS	ARG	ASN	MET	THR	VAL	HIS	PHE	LYS	VAL
4	LEU	ARG	GLU	GLU	GLY	PRO	ALA	HIS	MET	LYS
5	ASN	PHE	ILE	THR	ALA	CYS	ILE	VAL	GLY	SER
6	ILE	VAL	THR	GLU	GLY	GLU	GLY	ASN	GLY	LYS
7	LYS	VAL	SER	LYS	LYS	ARG	ALA	ALA	GLU	LYS
8	MET	LEU	VAL	GLU	LEU	GLN	LYS	LEU	PRO	PRO
9	LEU	THR	PRO	THR	LYS	GLU	PHE	ILE	VAL	ASP

1	GLY	SER	MET	ASP	GLU	GLY	ASP	LYS	LYS	SER
2	PRO	ILE	SER	GLN	VAL	HIS	GLU	ILE	GLY	ILE
3	LYS	ARG	ASN	MET	THR	VAL	HIS	PHE	LYS	VAL
4	LEU	ARG	GLU	GLU	GLY	PRO	ALA	HIS	MET	LYS
5	ASN	PHE	ILE	THR	ALA	CYS	ILE	VAL	GLY	SER
6	ILE	VAL	THR	GLU	GLY	GLU	GLY	ASN	GLY	LYS
7	LYS	VAL	SER	LYS	LYS	ARG	ALA	ALA	GLU	LYS
8	MET	LEU	VAL	GLU	LEU	GLN	LYS	LEU	PRO	PRO
9	LEU	THR	PRO	THR	LYS	GLU	PHE	ILE	VAL	ASP