BMRB Entry 4912
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4912
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Title: Structural Basis of Diverse Sequence-dependent Target Recognition by the 8kDa Dynein light chain PubMed: 11178896
Deposition date: 2000-12-06 Original release date: 2001-08-08
Authors: Fan, Jing-Song; Zhang, Qiang; Tochio, Hidehito; Li, Ming; Zhang, Mingjie
Citation: Fan, Jing-Song; Zhang, Qiang; Tochio, Hidehito; Li, Ming; Zhang, Mingjie. "Structural Basis of Diverse Sequence-dependent Target Recognition by the 8kDa Dynein light chain" J. Mol. Biol. 306, 97-108 (2001).
Assembly members:
dynein light chain 8, polymer, 89 residues, 10300 Da.
Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
dynein light chain 8: MCDRKAVIKNADMSEEMQQD
SVECATQALEKYNIEKDIAA
HIKKEFDKKYNPTWHCIVGR
NFGSYVTHETKHFIYFYLGQ
VAILLFKSG
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 616 |
| 13C chemical shifts | 248 |
| 15N chemical shifts | 96 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | DYNEIN light chain 8, DLC8, subunit a | 1 |
| 2 | DYNEIN light chain 8, DLC8, subunit b | 1 |
Entities:
Entity 1, DYNEIN light chain 8, DLC8, subunit a 89 residues - 10300 Da.
| 1 | MET | CYS | ASP | ARG | LYS | ALA | VAL | ILE | LYS | ASN | ||||
| 2 | ALA | ASP | MET | SER | GLU | GLU | MET | GLN | GLN | ASP | ||||
| 3 | SER | VAL | GLU | CYS | ALA | THR | GLN | ALA | LEU | GLU | ||||
| 4 | LYS | TYR | ASN | ILE | GLU | LYS | ASP | ILE | ALA | ALA | ||||
| 5 | HIS | ILE | LYS | LYS | GLU | PHE | ASP | LYS | LYS | TYR | ||||
| 6 | ASN | PRO | THR | TRP | HIS | CYS | ILE | VAL | GLY | ARG | ||||
| 7 | ASN | PHE | GLY | SER | TYR | VAL | THR | HIS | GLU | THR | ||||
| 8 | LYS | HIS | PHE | ILE | TYR | PHE | TYR | LEU | GLY | GLN | ||||
| 9 | VAL | ALA | ILE | LEU | LEU | PHE | LYS | SER | GLY |
Samples:
sample_1: dynein light chain 8, [U-13C;U-15N], 1.5 mM; phosphate buffer 100 mM; DTT 10 mM; D2O 99.99%
sample_2: dynein light chain 8, [U-15N], 1.5 mM; phosphate buffer 100 mM; DTT 10 mM; D2O 10%; H2O 90%
sample_3: dynein light chain 8, [U-15N; U-13C], 1.5 mM; phosphate buffer 100 mM; D2O 10%; H2O 90%
sample_cond_1: pH: 7.0; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | not available | not available | not available |
| 3D 15N-separated NOESY | not available | not available | not available |
| 3D 13C-separated NOESY | not available | not available | not available |
| HNHA | not available | not available | not available |
Software:
VNMR v6.1b - data collection
NMRPipe v1.7 - data processing
X-PLOR v3.8 - structure solution
NMR spectrometers:
- Varian INOVA 500 MHz
- Varian INOVA 750 MHz
Related Database Links:
| BMRB | 4305 |
| PDB | |
| DBJ | BAB22160 BAB27063 BAB27117 BAB28970 BAB28973 |
| EMBL | CAG28600 CAG46925 CAH65122 CAZ64801 CAZ64802 |
| GB | AAB04149 AAB38257 AAC32530 AAC32531 AAD01643 |
| REF | NP_001003901 NP_001032583 NP_001032584 NP_001075487 NP_001182043 |
| SP | P61273 P61285 P63167 P63168 P63169 |
| TPG | DAA17449 DAA20577 DAA23836 |
Download simulated HSQC data in one of the following formats:
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