BMRB Entry 4922
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4922
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Title: Backbone 1H, 13C and 15N chemical shift assignments for unfolded apoplastocyanin PubMed: 11316886
Deposition date: 2000-12-13 Original release date: 2001-08-08
Authors: Bai, Yawen; Chung, John; Dyson, Jane; Wright, Peter
Citation: Bai, Yawen; Chung, John; Dyson, H. Jane; Wright, Peter. "Structural and Dynamic Characterization of an Unfolded State of Poplar Apo-plastocyanin Formed under Non-denaturing Conditions" Protein Sci. 10, 1056-1066 (2001).
Assembly members:
unfolded apo-plastocyanin, polymer, 99 residues, Formula weight is not available
Natural source: Common Name: poplar Taxonomy ID: 3691 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Populus nigra
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
unfolded apo-plastocyanin: IDVLLGADDGSLAFVPSEFS
ISPGEKIVFKNNAGFPHNIV
FDEDSIPSGVDASKISMSEE
DLLNAKGETFEVALSNKGEY
SFYCSPHQGAGMVGKVTVN
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 304 |
| 13C chemical shifts | 270 |
| 15N chemical shifts | 92 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | unfolded apo-Pc | 1 |
Entities:
Entity 1, unfolded apo-Pc 99 residues - Formula weight is not available
| 1 | ILE | ASP | VAL | LEU | LEU | GLY | ALA | ASP | ASP | GLY | ||||
| 2 | SER | LEU | ALA | PHE | VAL | PRO | SER | GLU | PHE | SER | ||||
| 3 | ILE | SER | PRO | GLY | GLU | LYS | ILE | VAL | PHE | LYS | ||||
| 4 | ASN | ASN | ALA | GLY | PHE | PRO | HIS | ASN | ILE | VAL | ||||
| 5 | PHE | ASP | GLU | ASP | SER | ILE | PRO | SER | GLY | VAL | ||||
| 6 | ASP | ALA | SER | LYS | ILE | SER | MET | SER | GLU | GLU | ||||
| 7 | ASP | LEU | LEU | ASN | ALA | LYS | GLY | GLU | THR | PHE | ||||
| 8 | GLU | VAL | ALA | LEU | SER | ASN | LYS | GLY | GLU | TYR | ||||
| 9 | SER | PHE | TYR | CYS | SER | PRO | HIS | GLN | GLY | ALA | ||||
| 10 | GLY | MET | VAL | GLY | LYS | VAL | THR | VAL | ASN |
Samples:
sample_1: unfolded apo-plastocyanin, [U-15N], 2.0 mM; KH2PO4 5 mM; EDTA 50 uM; beta-mercaptoethanol 5 mM
sample_2: unfolded apo-plastocyanin, [U-15N; U-13C], 1.5 mM; KH2PO4 5 mM; EDTA 50 uM; beta-mercaptoethanol 5 mM
sample_3: unfolded apo-plastocyanin, [U-15N; U-13C], 0.5 mM; KH2PO4 5 mM; EDTA 50 uM; beta-mercaptoethanol 5 mM
sample_4: unfolded apo-plastocyanin 0.1 mM; KH2PO4 5 mM; EDTA 50 uM; beta-mercaptoethanol 5 mM
Ex-cond_1: pH: 6.0; temperature: 308 K; ionic strength: 5 mM
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N NOESY-HSQC | not available | not available | not available |
| C(CO)NH-TOCSY | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| TOCSY-HSQC | not available | not available | not available |
| HNCACB | not available | not available | not available |
| HNCA | not available | not available | not available |
| HNCO | not available | not available | not available |
| (HA)CO(CA)NH(particularly useful for unfolded proteins) | not available | not available | not available |
Software:
FELIX - Fourier transformation, analysis
NMR spectrometers:
- Bruker AMX 500 MHz
Related Database Links:
| BMRB | 19247 4019 |
| PDB | |
| EMBL | CAA90564 |
| GB | ABK96770 EEE94750 |
| REF | XP_002307754 XP_011003094 |
| SP | P00299 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts