BMRB Entry 4923
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4923
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Title: 1H chemical shift assignments for omega-atracotoxin-Hv2a PubMed: 11522785
Deposition date: 2000-12-13 Original release date: 2002-09-23
Authors: Wang, Xiu-hong; King, Glenn
Citation: Wang, Xiu-hong; Connor, M.; Wilson, D.; Wilson, H.; Nicholson, G.; Smith, R.; Shaw, D.; Mackay, J.; Alewood, P.; Christie, M.; King, Glenn. "Discovery and structure of a potent and highly specific blocker of insect calcium channels." J. Biol. Chem. 276, 40306-40312 (2001).
Assembly members:
omega-atracotoxin-Hv2a, polymer, 45 residues, 4478 Da.
Natural source: Common Name: Blue Mountains funnel-web spider Taxonomy ID: 6904 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Hadronyche versuta
Experimental source: Production method: purified from the natural source
Entity Sequences (FASTA):
omega-atracotoxin-Hv2a: LLACLFGNGRCSSNRDCCEL
TPVCKRGSCVSSGPGLVGGI
LGGIL
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 280 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | omega-ACTX-Hv2a monomer | 1 |
Entities:
Entity 1, omega-ACTX-Hv2a monomer 45 residues - 4478 Da.
| 1 | LEU | LEU | ALA | CYS | LEU | PHE | GLY | ASN | GLY | ARG | ||||
| 2 | CYS | SER | SER | ASN | ARG | ASP | CYS | CYS | GLU | LEU | ||||
| 3 | THR | PRO | VAL | CYS | LYS | ARG | GLY | SER | CYS | VAL | ||||
| 4 | SER | SER | GLY | PRO | GLY | LEU | VAL | GLY | GLY | ILE | ||||
| 5 | LEU | GLY | GLY | ILE | LEU |
Samples:
omega-Hv2a_sample_1: omega-atracotoxin-Hv2a 1.6 mM; chloramphenicol 25 microM; TSP 0.1 mM; D2O 5%
Ex-cond_1: ionic strength: 0.005 M; pH: 4.71; temperature: 288 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | not available | not available | not available |
| 2D 1H-1H NOESY | not available | not available | not available |
| 2D 1H-1H DQF-COSY | not available | not available | not available |
| 2D 1H-1H E.COSY | not available | not available | not available |
Software:
XEASY v1.3.13 - spectral analysis
NMR spectrometers:
- Bruker AVANCE 600 MHz