BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5051

Title: Backbone 1H, 13C, and 15N and Side-Chain 1H Chemical Shift Assignments for MTH1692   PubMed: 11854485

Deposition date: 2001-06-11 Original release date: 2002-08-22

Authors: Yee, Adelinda; Chang, X.; Pineda_Lucena, A.; Wu, B.; Semesi, A.; Le, B.; Ramelot, T.; Lee, G.; Bhattacharyya, S.; Gutierrez, P.; Denisov, A.; Lee, C.; Cort, J.; Kozlov, G.; Liao, J.; Finak, G.; Chen, L.; Wishart, D.; Lee, W.; McIntosh, L.; Gehring, Kalle; Kennedy, M.; Edwards, A.; Arrowsmith, Cheryl

Citation: Yee, Adelinda; Chang, X.; Pineda_Lucena, A.; Wu, B.; Semesi, A.; Le, B.; Ramelot, T.; Lee, G.; Bhattacharyya, S.; Gutierrez, P.; Denisov, A.; Lee, C.; Cort, J.; Kozlov, G.; Liao, J.; Finak, G.; Chen, L.; Wishart, D.; Lee, W.; McIntosh, L.; Gehring, Kalle; Kennedy, M.; Edwards, A.; Arrowsmith, Cheryl. "An NMR Approach to Stuctural Proteomics"  Proc. Natl. Acad. Sci. U.S.A. 99, 1825-1830 (2002).

Assembly members:
MTH1692, polymer, 208 residues, 22614 Da.

Natural source:   Common Name: Methanothermobacter thermautotrophicus   Taxonomy ID: 145262   Superkingdom: Archaea   Kingdom: Euryarchaeota   Genus/species: Methanobacterium thermoautotrophicum

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
MTH1692: MLIRKITRKNPSPDVLEEAI SVMEGGGIVIYPTDTIYGLG VNALDEDAVRRLFRVKGRSP HKPVSICVSCVDEIPRFSRP SGDAMELMERILPGPYTVVL ERNELIPDVITGGSSRVGIR VPDDEICRRIAARFPVTATS ANISGKPPSPRLEEIVRDLD AVDLVLDAGDCLDMEPSTVI DLTVNPPRVLRRGKGPLDPV LLRGAGDV

Data sets:
Data typeCount
1H chemical shifts1141
13C chemical shifts309
15N chemical shifts175

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MTH16921

Entities:

Entity 1, MTH1692 208 residues - 22614 Da.

1   METLEUILEARGLYSILETHRARGLYSASN
2   PROSERPROASPVALLEUGLUGLUALAILE
3   SERVALMETGLUGLYGLYGLYILEVALILE
4   TYRPROTHRASPTHRILETYRGLYLEUGLY
5   VALASNALALEUASPGLUASPALAVALARG
6   ARGLEUPHEARGVALLYSGLYARGSERPRO
7   HISLYSPROVALSERILECYSVALSERCYS
8   VALASPGLUILEPROARGPHESERARGPRO
9   SERGLYASPALAMETGLULEUMETGLUARG
10   ILELEUPROGLYPROTYRTHRVALVALLEU
11   GLUARGASNGLULEUILEPROASPVALILE
12   THRGLYGLYSERSERARGVALGLYILEARG
13   VALPROASPASPGLUILECYSARGARGILE
14   ALAALAARGPHEPROVALTHRALATHRSER
15   ALAASNILESERGLYLYSPROPROSERPRO
16   ARGLEUGLUGLUILEVALARGASPLEUASP
17   ALAVALASPLEUVALLEUASPALAGLYASP
18   CYSLEUASPMETGLUPROSERTHRVALILE
19   ASPLEUTHRVALASNPROPROARGVALLEU
20   ARGARGGLYLYSGLYPROLEUASPPROVAL
21   LEULEUARGGLYALAGLYASPVAL

Samples:

sample_1: MTH1692, [U-15N], 3.0 mM; NaCl 150 mM

sample_2: MTH1692, [U-13C; U-15N], 3.0 mM; NaCl 150 mM

sample_3: MTH1692 3.0 mM; NaCl 150 mM

conditions_1: pH: 6.0; temperature: 320 K; ionic strength: 0.15 M

Experiments:

NameSampleSample stateSample conditions
3D CBCA(CO)NHnot availablenot availablenot available
3D HNCACBnot availablenot availablenot available
3D HNCAnot availablenot availablenot available
3D 1H-1H-15N NOESYnot availablenot availablenot available
3D 1H-1H-15N TOCSYnot availablenot availablenot available
3D 13C-1H-1H NOESYnot availablenot availablenot available
2D 1H-1H NOESYnot availablenot availablenot available

Software:

XWINNMR v2.1 - data collection

GIFA v4.31 - data processing

XEASY v1.3.13 - data analysis

ARIA v0.9 - automated NOE assignment

CNS v0.9 - refinement

NMR spectrometers:

  • Varian UnityPlus 800 MHz
  • Bruker DRX 500 MHz

Related Database Links:

PDB
DBJ BAM70792
GB AAB86164
REF NP_276803 WP_010877299

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts