BMRB Entry 5155
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5155
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Title: NMR structure of the UBX domain from P47 (energy minimised average) PubMed: 11478859
Deposition date: 2001-09-17 Original release date: 2001-10-18
Authors: Yuan, X.; Shaw, A.; Zhang, X.; Kondo, H.; Lally, J.; Freemont, P.; Matthews, S.
Citation: Yuan, X.; Shaw, A.; Zhang, X.; Kondo, H.; Lally, J.; Freemont, P.; Matthews, S.. "Solution Structure and Interaction Surface of the C-terminal Domain from p47: A Major p97-cofactor Involved in SNARE Disassembly " J. Mol. Biol. 311, 255-263 (2001).
Assembly members:
p47, polymer, 89 residues, Formula weight is not available
Natural source: Common Name: Norway rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
p47: KASSSILINEAEPTTNIQIR
LADGGRLVQKFNHSHRISDI
RLFIVDARPAMAATSFVLMT
TFPNKELADENQTLKEANLL
NAVIVQRLT
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 607 |
| 13C chemical shifts | 174 |
| 15N chemical shifts | 86 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | p47 protein | 1 |
Entities:
Entity 1, p47 protein 89 residues - Formula weight is not available
| 1 | LYS | ALA | SER | SER | SER | ILE | LEU | ILE | ASN | GLU | ||||
| 2 | ALA | GLU | PRO | THR | THR | ASN | ILE | GLN | ILE | ARG | ||||
| 3 | LEU | ALA | ASP | GLY | GLY | ARG | LEU | VAL | GLN | LYS | ||||
| 4 | PHE | ASN | HIS | SER | HIS | ARG | ILE | SER | ASP | ILE | ||||
| 5 | ARG | LEU | PHE | ILE | VAL | ASP | ALA | ARG | PRO | ALA | ||||
| 6 | MET | ALA | ALA | THR | SER | PHE | VAL | LEU | MET | THR | ||||
| 7 | THR | PHE | PRO | ASN | LYS | GLU | LEU | ALA | ASP | GLU | ||||
| 8 | ASN | GLN | THR | LEU | LYS | GLU | ALA | ASN | LEU | LEU | ||||
| 9 | ASN | ALA | VAL | ILE | VAL | GLN | ARG | LEU | THR |
Samples:
sample_1: p47, [U-13C; U-15N], 1 mM; NaAc 20 mM; H2O 90%; D2O 10%
sample_cond_1: pH: 5.2; temperature: 302 K; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCA | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HBHA(CBCACO)NH | not available | not available | not available |
| 3D 15N-separated NOESY | not available | not available | not available |
Software:
AURELIA v2.1.5 - data analysis
NMRPipe - processing
XWINNMR - collection
NMRView v3.1.1 - data analysis
X-PLOR v3.853 - structure solution, refinement
NMR spectrometers:
- Bruker DRX 500 MHz
Related Database Links:
| BMRB | 5876 |
| PDB | |
| DBJ | BAA21659 BAB28604 BAE23234 |
| EMBL | CAA71742 |
| GB | AAH50936 AAH72464 AAI06102 EDL05910 EDL05911 |
| REF | NP_001278003 NP_114187 NP_938085 XP_003500001 XP_003500002 |
| SP | O35987 Q9CZ44 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts