BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5197

Title: 1H and 13CA chemical shift assignments of the polypeptide mPrP(143-158)   PubMed: 10397798

Deposition date: 2001-10-29 Original release date: 2001-11-12

Authors: Liu, Aizhuo; Riek, Roland; Zahn, Ralph; Glockshuber, Rudolf; Wuthrich, Kurt

Citation: Liu, Aizhuo; Riek, Roland; Zahn, Ralph; Glockshuber, Rudolf; Wuthrich, Kurt. "Helix protensity of a polypeptide containing helix 1 of the mouse prion protein studied by NMR and CD spectroscopy "  Biopolymers 51, 145-152 (1999).

Assembly members:
mPrP(143-158), polymer, 16 residues, Formula weight is not available

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
mPrP(143-158): NDWEDRYYRENMYRYP

Data typeCount
1H chemical shifts604
13C chemical shifts64

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1mouse prion protein, trans conformer1
2mouse prion protein, cis conformer1

Entities:

Entity 1, mouse prion protein, trans conformer 16 residues - Formula weight is not available

1   ASNASPTRPGLUASPARGTYRTYRARGGLU
2   ASNMETTYRARGTYRPRO

Samples:

sample_1: mPrP(143-158) 1.0 mM; H2O 90%; D2O 10%

sample_2: mPrP(143-158) 1.0 mM; H2O 90%; TFE, [U-2H], 10%

sample_3: mPrP(143-158) 1.0 mM; H2O 75%; TFE, [U-2H], 25%

sample_4: mPrP(143-158) 1.0 mM; H2O 50%; TFE, [U-2H], 50%

Ex-cond_1: pH: 4.5; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
not availablenot availablenot availableEx-cond_1

Software:

No software information available

NMR spectrometers:

  • Varian INOVA 400 MHz
  • Bruker DRX 500 MHz

Related Database Links:

BMRB 15845 16071 16075 16076 16077 16078 16079 16080 16184 16185 16722 16723 17081 17082 17084 17087 17174 17213 17758 17759
PDB
DBJ BAA08790 BAE28320 BAE28693 BAE29994 BAE34221
EMBL CAJ18553
GB AAA39996 AAA39997 AAA39998 AAA39999 AAA41947
PRF 1106228A
REF NP_001265185 NP_035300 NP_036763 XP_006235124
SP P04925 P13852 Q9Z0T3