BMRB Entry 5200
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5200
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Title: Sequence-specific 1H, 13C and 15N chemical shift assignment of DnaA domain IV PubMed: 12435387
Deposition date: 2001-11-04 Original release date: 2003-06-10
Authors: Imoto, Taiji
Citation: Obita, Takayuki; Iwura, Takafumi; Su'etsugu, Masayuki; Yoshida, Yoichiro; Tanaka, Yoshitsugu; Katayama, Tsutomu; Ueda, Tadashi; Imoto, Taiji. "Determination of the Secondary Structure in Solution of the Escherichia coli DnaA DNA-binding Domain " Biochem. Biophys. Res. Commun. 299, 42-48 (2002).
Assembly members:
chromosome replication initiator, polymer, 95 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
chromosome replication initiator: MVTIDNIQKTVAEYYKIKVA
DLLSKRRSRSVARPRQMAMA
LAKELTNHSLPEIGDAFGGR
DHTTVLHACRKIEQLREESH
DIKEDFSNLIRTLSS
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 165 |
| 13C chemical shifts | 274 |
| 15N chemical shifts | 89 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | DnaA domain IV | 1 |
Entities:
Entity 1, DnaA domain IV 95 residues - Formula weight is not available
| 1 | MET | VAL | THR | ILE | ASP | ASN | ILE | GLN | LYS | THR | ||||
| 2 | VAL | ALA | GLU | TYR | TYR | LYS | ILE | LYS | VAL | ALA | ||||
| 3 | ASP | LEU | LEU | SER | LYS | ARG | ARG | SER | ARG | SER | ||||
| 4 | VAL | ALA | ARG | PRO | ARG | GLN | MET | ALA | MET | ALA | ||||
| 5 | LEU | ALA | LYS | GLU | LEU | THR | ASN | HIS | SER | LEU | ||||
| 6 | PRO | GLU | ILE | GLY | ASP | ALA | PHE | GLY | GLY | ARG | ||||
| 7 | ASP | HIS | THR | THR | VAL | LEU | HIS | ALA | CYS | ARG | ||||
| 8 | LYS | ILE | GLU | GLN | LEU | ARG | GLU | GLU | SER | HIS | ||||
| 9 | ASP | ILE | LYS | GLU | ASP | PHE | SER | ASN | LEU | ILE | ||||
| 10 | ARG | THR | LEU | SER | SER |
Samples:
sample_1: chromosome replication initiator 2.0 mM
Ex-cond_1: pH: 6.0; temperature: 303 K; ionic strength: 0.1 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N NOESY | not available | not available | not available |
Software:
NMRPipe -
NMR spectrometers:
- Varian Inova 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAB38060 BAE73276 BAE77592 BAG79512 BAH65893 |
| EMBL | CAD03157 CAE12296 CAG77337 CAH23181 CAL14189 |
| GB | AAA02815 AAA02924 AAA83958 AAB59149 AAC76725 |
| PIR | AI0957 |
| REF | NP_290335 NP_312664 NP_418157 NP_458104 NP_462738 |
| SP | A1JT80 A4TGL5 A7FPB7 A7ZTQ8 A8A6G3 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts