BMRB Entry 5241
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5241
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Title: 1H and 15N assignment and secondary structure of the double K18G/R82E mutant of Alicyclobacillus acidocaldarius thermostable thioredoxin PubMed: 15147188
Deposition date: 2001-12-20 Original release date: 2004-09-10
Authors: Leone, Marilisa; Di Lello, Paola; Pedone, Emilia; Bartolucci, Simonetta; Rossi, Mose'; Di Blasio, Benedetto; Pedone, Carlo; Saviano, Michele; Isernia, Carla; Fattorusso, Roberto
Citation: Leone, Marilisa; Di Lello, Paola; Ohlenschlager, O.; Pedone, Emilia; Bartolucci, Simonetta; Rossi, Mose'; Di Blasio, Benedetto; Pedone, Carlo; Saviano, Michele; Isernia, Carla; Fattorusso, Roberto. "Solution structure and backbone dynamics of the K18G/R82E Alicyclobacillus acidocaldarius thioredoxin mutant: a molecular analysis of its reduced thermal stability." Biochemistry 43, 6043-6058 (2004).
Assembly members:
Alicyclobacillus acidocaldarius thioredoxin, polymer, 105 residues, 11475.0 Da.
Natural source: Common Name: Alicyclobacillus acidocaldarius (Bacillus acidocaldarius) Taxonomy ID: 1388 Superkingdom: Eubacteria Kingdom: not available Genus/species: Alicyclobacillus acidocaldarius
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Alicyclobacillus acidocaldarius thioredoxin: ATMTLTDANFQQAIQGDGPV
LVDFWAAWCGPCRMMAPVLE
EFAEAHADKVTVAKLNVDEN
PETTSQFGIMSIPTLILFKG
GEPVKQLIGYQPKEQLEAQL
ADVLQ
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 756 |
| 15N chemical shifts | 111 |
Additional metadata:
Related Database Links:
| BMRB | 4446 5240 |
| PDB | 1NSW 1NW2 1QUW 1RQM |
| GB | ACV57898 AEJ42820 |
| REF | WP_012810252 WP_014463721 YP_003184287 YP_005517339 |
| SP | P80579 |
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