BMRB Entry 5266

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5266

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: 1H, 15N and 13C assignments of the focal adhesion targeting domain of focal adhesion kinase (FAT) PubMed: 12061723

Deposition date: 2002-01-28 Original release date: 2003-01-06

Authors: Liu, Gaohua; Guibao, Cristina; Zheng, Jie

Citation: Liu, Gaohua; Guibao, Cristina; Zheng, Jie. "Letter to the Editor: 1H, 15N and 13C Assignments of the Targeting (FAT) Domain of Focal Adhesion Kinase " J. Biomol. NMR 23, 75-76 (2002).

Assembly members:
Focal Adhesion Targeting domain of focal adhesion kinase (914-1053), polymer, 139 residues, Formula weight is not available

Natural source: Common Name: Chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):
Focal Adhesion Targeting domain of focal adhesion kinase (914-1053): MANLDRSNDKVYENVTGLVK AVIEMSSKIQPAPPEEYVPM VKEVGLALRTLLATVDESLP VLPASTHREIEMAQKLLNSD LAELINKMKLAQQYVMTSLQ QEYKKQMLTAAHALAVDAKN LLDVIDQARLKMISQSRPH

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	125
13C chemical shifts	270
15N chemical shifts	125

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FAT domain (914-1053)	1

Entities:

Entity 1, FAT domain (914-1053) 139 residues - Formula weight is not available

1

MET

ALA

ASN

LEU

ASP

ARG

SER

ASN

ASP

LYS

2

VAL

TYR

GLU

ASN

VAL

THR

GLY

LEU

VAL

LYS

3

ALA

VAL

ILE

GLU

MET

SER

LYS

ILE

GLN

4

PRO

ALA

PRO

GLU

TYR

VAL

PRO

MET

5

VAL

LYS

GLU

VAL

GLY

LEU

ALA

LEU

ARG

THR

6

LEU

ALA

THR

VAL

ASP

GLU

SER

LEU

PRO

7

VAL

LEU

PRO

ALA

SER

THR

HIS

ARG

GLU

ILE

8

GLU

MET

ALA

GLN

LYS

LEU

ASN

SER

ASP

9

LEU

ALA

GLU

LEU

ILE

ASN

LYS

MET

LYS

LEU

10

ALA

GLN

TYR

VAL

MET

THR

SER

LEU

GLN

11

GLN

GLU

TYR

LYS

GLN

MET

LEU

THR

ALA

12

ALA

HIS

ALA

LEU

ALA

VAL

ASP

ALA

LYS

ASN

13

LEU

ASP

VAL

ILE

ASP

GLN

ALA

ARG

LEU

14

LYS

MET

ILE

SER

GLN

SER

ARG

PRO

HIS

Samples:

sample_1: Focal Adhesion Targeting domain of focal adhesion kinase (914-1053), [U-15N], 1.0 mL

sample_2: Focal Adhesion Targeting domain of focal adhesion kinase (914-1053), [U-13C; U-15N], 2.0 mL

Ex-cond_1: pH: 6.2 na; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HAQC	not available	not available	not available
1H-15N NOESYHSQC	not available	not available	not available
1H-15N TOCSYHSQC	not available	not available	not available
HNCA	not available	not available	not available
HNCACB	not available	not available	not available
CBCACONH	not available	not available	not available
HN(CO)CA	not available	not available	not available
3D C13-edit NOESY	not available	not available	not available
3D HCCHTOCSY	not available	not available	not available
3D HCCHCOSY	not available	not available	not available
4D 15N-C13 NOESY	not available	not available	not available
4D C13-C13 NOESY	not available	not available	not available

Software:

XEASY v1.3.13 -

NMR spectrometers:

Varian INOVA 600 MHz

BMRB	5677 5924
PDB	1KTM 1PV3 1QVX 2L6F 2L6G 2L6H
GB	AAA48765 AAA48773 EMC80181 EOA99770 KFM11460
REF	NP_990766 XP_002191290 XP_004174254 XP_005021656 XP_005021657
SP	Q00944

Biological Magnetic Resonance Data Bank