BMRB Entry 5294
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5294
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Title: Structure of the beta subunit of translation initiation factor 2 from the archaeon Methanococcus jannaschii: A representative of the eIF2beta/eIF5 family of proteins PubMed: 11980477
Deposition date: 2002-02-18 Original release date: 2002-09-11
Authors: Cho, Seongeun; Hoffman, David
Citation: Cho, Seongeun; Hoffman, David. "Structure of the Beta Subunit of Translation Initiation Factor 2 from the Archaeon Methanococcus jannaschii: A Representative of the eIF2beta/eIF5 Family of Proteins " Biochemistry 41, 5730-5742 (2002).
Assembly members:
translation initiation factor IF2b, polymer, 143 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Methanococcus jannaschii Taxonomy ID: 2190 Superkingdom: Archaea Kingdom: not available Genus/species: Methanococcus jannaschii
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
translation initiation factor IF2b: MSDLENIDYYDYKALLKRAR
SQIPDYVFQKDRFELPEIEI
LIEGNRTIIRNFRELAKAVN
RDEEFFAKYLLKETGSAGNL
EGGRLILQRRISPELLKSRI
NDFLREYVICRECGKPDTKI
IKEGRVHLLKCMACGAIRPI
RMI
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 485 |
| 13C chemical shifts | 181 |
| 15N chemical shifts | 103 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | aIF2b | 1 |
| 2 | ZINC (II) ION | 2 |
Entities:
Entity 1, aIF2b 143 residues - Formula weight is not available
| 1 | MET | SER | ASP | LEU | GLU | ASN | ILE | ASP | TYR | TYR | ||||
| 2 | ASP | TYR | LYS | ALA | LEU | LEU | LYS | ARG | ALA | ARG | ||||
| 3 | SER | GLN | ILE | PRO | ASP | TYR | VAL | PHE | GLN | LYS | ||||
| 4 | ASP | ARG | PHE | GLU | LEU | PRO | GLU | ILE | GLU | ILE | ||||
| 5 | LEU | ILE | GLU | GLY | ASN | ARG | THR | ILE | ILE | ARG | ||||
| 6 | ASN | PHE | ARG | GLU | LEU | ALA | LYS | ALA | VAL | ASN | ||||
| 7 | ARG | ASP | GLU | GLU | PHE | PHE | ALA | LYS | TYR | LEU | ||||
| 8 | LEU | LYS | GLU | THR | GLY | SER | ALA | GLY | ASN | LEU | ||||
| 9 | GLU | GLY | GLY | ARG | LEU | ILE | LEU | GLN | ARG | ARG | ||||
| 10 | ILE | SER | PRO | GLU | LEU | LEU | LYS | SER | ARG | ILE | ||||
| 11 | ASN | ASP | PHE | LEU | ARG | GLU | TYR | VAL | ILE | CYS | ||||
| 12 | ARG | GLU | CYS | GLY | LYS | PRO | ASP | THR | LYS | ILE | ||||
| 13 | ILE | LYS | GLU | GLY | ARG | VAL | HIS | LEU | LEU | LYS | ||||
| 14 | CYS | MET | ALA | CYS | GLY | ALA | ILE | ARG | PRO | ILE | ||||
| 15 | ARG | MET | ILE |
Entity 2, ZINC (II) ION - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: translation initiation factor IF2b, [U-13C; U-15N], 0.5 – 1.5 mM; ZINC (II) ION mM
sample_2: translation initiation factor IF2b, [U-15N], 0.5 – 1.5 mM; ZINC (II) ION mM
condition_1: pH: 6.0 na; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCA | not available | not available | condition_1 |
| HNCO | not available | not available | condition_1 |
| HNCACB | not available | not available | condition_1 |
| HN(CO)CACB | not available | not available | condition_1 |
| 3D-HCCH-TOCSY | not available | not available | condition_1 |
| 15N-HMQC | not available | not available | condition_1 |
| 3D 1H-1H-15N NOESY | not available | not available | condition_1 |
| 3D 1H-13C-1H HCCH-TOCSY | not available | not available | condition_1 |
Software:
felix - processing
NMR spectrometers:
- Varian INOVA 500 MHz
Related Database Links:
| GB | AAB98077 ACV24219 ADC69390 AIJ05339 |
| REF | NP_247061 WP_010869589 WP_012980300 WP_015790959 YP_003127719 |
| SP | Q57562 |
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