BMRB Entry 5323
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5323
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Title: Solution Structure of the Tenebrio molitor Antifreeze Protein PubMed: 11969412
Deposition date: 2002-03-16 Original release date: 2002-05-10
Authors: Daley, M.; Spyracopoulos, L.; Jia, Z.; Davies, P.; Sykes, B.
Citation: Daley, M.; Spyracopoulos, L.; Jia, Z.; Davies, P.; Sykes, B.. "Structure and Dynamics of a beta-Helical Antifreeze Protein" Biochemistry 41, 5515-5525 (2002).
Assembly members:
Tenebrio molitor antifreeze protein, polymer, 84 residues, Formula weight is not available
Natural source: Common Name: yellow mealworm beetle Taxonomy ID: 7067 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Tenebrio molitor
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Tenebrio molitor antifreeze protein: QCTGGADCTSCTGACTGCGN
CPNAVTCTNSQHCVKANTCT
GSTDCNTAQTCTNSKDCFEA
NTCTDSTNCYKATACTNSSG
CPGH
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 85 |
| 1H chemical shifts | 420 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Thermal hysteresis protein isoform YL-1 (2-14) | 1 |
Entities:
Entity 1, Thermal hysteresis protein isoform YL-1 (2-14) 84 residues - Formula weight is not available
| 1 | GLN | CYS | THR | GLY | GLY | ALA | ASP | CYS | THR | SER | ||||
| 2 | CYS | THR | GLY | ALA | CYS | THR | GLY | CYS | GLY | ASN | ||||
| 3 | CYS | PRO | ASN | ALA | VAL | THR | CYS | THR | ASN | SER | ||||
| 4 | GLN | HIS | CYS | VAL | LYS | ALA | ASN | THR | CYS | THR | ||||
| 5 | GLY | SER | THR | ASP | CYS | ASN | THR | ALA | GLN | THR | ||||
| 6 | CYS | THR | ASN | SER | LYS | ASP | CYS | PHE | GLU | ALA | ||||
| 7 | ASN | THR | CYS | THR | ASP | SER | THR | ASN | CYS | TYR | ||||
| 8 | LYS | ALA | THR | ALA | CYS | THR | ASN | SER | SER | GLY | ||||
| 9 | CYS | PRO | GLY | HIS |
Samples:
sample_1: Tenebrio molitor antifreeze protein, [U-15N], 0.4 mM; H2O 90%; D2O 10%
sample_2: Tenebrio molitor antifreeze protein 1.0 mM; H2O 90%; D2O 10%
sample_3: Tenebrio molitor antifreeze protein 1.0 mM; D2O 100%
sample_cond_1: ionic strength: 0 mM; pH: 5.5; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-edited TOCSY | not available | not available | not available |
| HNHA | not available | not available | not available |
| HNHB | not available | not available | not available |
| 3D 15N-edited NOESY | not available | not available | not available |
| 2D TOCSY | not available | not available | not available |
| 2D NOESY | not available | not available | not available |
| 13C-HSQC | not available | not available | not available |
| 15N-HSQC | not available | not available | not available |
Software:
VNMR v6.1B - collection
NMRPipe vsgi6 - processing
NMRView v4.1.2 - data analysis
CNS v1.0 - structure solution
NMR spectrometers:
- Varian Unity 600 MHz
- Varian INOVA 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts