BMRB Entry 5406
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5406
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Human Cytochrome c PubMed: 12678434
Deposition date: 2002-06-27 Original release date: 2003-07-30
Authors: Jeng, Wen-Yih; Chuang, Woei-Jer
Citation: Jeng, Wen-Yih; Chen, Chiu-Yueh; Chang, Hsien-Chang; Chuang, Woei-Jer. "Expression and Characterization of Recombinant Human Cytochrome c in E. coli" J. Bioenerg. Biomembr. 34, 423-431 (2002).
Assembly members:
cytochrome c, polymer, 104 residues, 12234 Da.
HEC, non-polymer, 618.503 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
cytochrome c: GDVEKGKKIFIMKCSQCHTV
EKGGKHKTGPNLHGLFGRKT
GQAPGYSYTAANKNKGIIWG
EDTLMEYLENPKKYIPGTKM
IFVGIKKKEERADLIAYLKK
ATNE
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 756 |
| 13C chemical shifts | 219 |
| 15N chemical shifts | 103 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | cytochrome c peptide | 1 |
| 2 | heme c | 2 |
Entities:
Entity 1, cytochrome c peptide 104 residues - 12234 Da.
| 1 | GLY | ASP | VAL | GLU | LYS | GLY | LYS | LYS | ILE | PHE | ||||
| 2 | ILE | MET | LYS | CYS | SER | GLN | CYS | HIS | THR | VAL | ||||
| 3 | GLU | LYS | GLY | GLY | LYS | HIS | LYS | THR | GLY | PRO | ||||
| 4 | ASN | LEU | HIS | GLY | LEU | PHE | GLY | ARG | LYS | THR | ||||
| 5 | GLY | GLN | ALA | PRO | GLY | TYR | SER | TYR | THR | ALA | ||||
| 6 | ALA | ASN | LYS | ASN | LYS | GLY | ILE | ILE | TRP | GLY | ||||
| 7 | GLU | ASP | THR | LEU | MET | GLU | TYR | LEU | GLU | ASN | ||||
| 8 | PRO | LYS | LYS | TYR | ILE | PRO | GLY | THR | LYS | MET | ||||
| 9 | ILE | PHE | VAL | GLY | ILE | LYS | LYS | LYS | GLU | GLU | ||||
| 10 | ARG | ALA | ASP | LEU | ILE | ALA | TYR | LEU | LYS | LYS | ||||
| 11 | ALA | THR | ASN | GLU |
Entity 2, heme c - C34 H34 Fe N4 O4 - 618.503 Da.
| 1 | HEC |
Samples:
sample_1: cytochrome c 2 mM
sample_2: cytochrome c, [U-15N], 2 mM
sample_3: cytochrome c, [U-13C; U-15N], 2 mM
sample_4: cytochrome c, selective 15N-G, 0.5 mM
cond._set_1: pH: 6.5; temperature: 300 K; ionic strength: 0.1 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-1H NOESY | not available | not available | cond._set_1 |
| 1H-1H TOCSY | not available | not available | cond._set_1 |
| 1H-15N HSQC | not available | not available | cond._set_1 |
| 1H-1H-15N NOESY | not available | not available | cond._set_1 |
| 1H-1H-15N TOCSY | not available | not available | cond._set_1 |
| 13C-1H-1H NOESY | not available | not available | cond._set_1 |
| 1H-13C-1H HCCH-TOCSY | not available | not available | cond._set_1 |
| 1H-13C-1H HCCH-COSY | not available | not available | cond._set_1 |
| 1H-13C-1H HCCH-NOESY | not available | not available | cond._set_1 |
| HNCACB | not available | not available | cond._set_1 |
| CBCA(CO)NH | not available | not available | cond._set_1 |
| HBHA(CBCA)NH | not available | not available | cond._set_1 |
| HBHA(CBCACO)NH | not available | not available | cond._set_1 |
Software:
xwinnmr v2.6 -
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts