BMRB Entry 5467
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5467
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: 1H,15N and 13C resonance assignments of the SH3-like domain of the human ADAP protein PubMed: 15062083
Deposition date: 2002-07-19 Original release date: 2004-10-13
Authors: Gupta, Prateek; Langdon, Grant; Kofler, Michael; Schmidt, Stefanie; Heuer, Katja; Thiemke, Katharina; Freund, Christian
Citation: Heuer, Katja; Kofler, Michael; Langdon, Grant; Thiemke, Katharina; Freund, Christian. "Structure of a helically extended SH3 domain of the T cell adapter protein ADAP" Structure (Cambridge, MA, U. S.) 12, 603-610 (2004).
Assembly members:
SH3 like domain of ADAP-binding protein, polymer, 102 residues, 11873.5 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
SH3 like domain of ADAP-binding protein: MGSSHHHHHHSSGKKLKKQE
KEEKDFRKKFKYDGEIRVLY
STKVTTSITSKKWGTRDLQV
KPGESLEVIQTTDDTKVLCR
NEEGKYGYVLRSYLADNDGE
IY
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 345 |
15N chemical shifts | 87 |
1H chemical shifts | 560 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | SH3 like domain of ADAP protein | 1 |
Entities:
Entity 1, SH3 like domain of ADAP protein 102 residues - 11873.5 Da.
1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
2 | SER | SER | GLY | LYS | LYS | LEU | LYS | LYS | GLN | GLU | ||||
3 | LYS | GLU | GLU | LYS | ASP | PHE | ARG | LYS | LYS | PHE | ||||
4 | LYS | TYR | ASP | GLY | GLU | ILE | ARG | VAL | LEU | TYR | ||||
5 | SER | THR | LYS | VAL | THR | THR | SER | ILE | THR | SER | ||||
6 | LYS | LYS | TRP | GLY | THR | ARG | ASP | LEU | GLN | VAL | ||||
7 | LYS | PRO | GLY | GLU | SER | LEU | GLU | VAL | ILE | GLN | ||||
8 | THR | THR | ASP | ASP | THR | LYS | VAL | LEU | CYS | ARG | ||||
9 | ASN | GLU | GLU | GLY | LYS | TYR | GLY | TYR | VAL | LEU | ||||
10 | ARG | SER | TYR | LEU | ALA | ASP | ASN | ASP | GLY | GLU | ||||
11 | ILE | TYR |
Samples:
Sample_1: SH3 like domain of ADAP-binding protein, [U-95% 13C; U-90% 15N], 0.5 1.8 mM
Ex-cond_1: ionic strength: 0.05 M; pH: 6.3; temperature: 300 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
1H-15N HSQC | Sample_1 | not available | Ex-cond_1 |
CBCACONH | Sample_1 | not available | Ex-cond_1 |
CBCANH | Sample_1 | not available | Ex-cond_1 |
HBHACONH | Sample_1 | not available | Ex-cond_1 |
HNCO | Sample_1 | not available | Ex-cond_1 |
1H-15N NOESY | Sample_1 | not available | Ex-cond_1 |
1H-15N TOCSY | Sample_1 | not available | Ex-cond_1 |
1H-13C TOCSY | Sample_1 | not available | Ex-cond_1 |
1H-13C NOESY | Sample_1 | not available | Ex-cond_1 |
1H-1H TOCSY | Sample_1 | not available | Ex-cond_1 |
1H-1H NOESY | Sample_1 | not available | Ex-cond_1 |
Software:
SPARKY v3.106 - semi automated peak assignments
XEASY v1.3.13 - peak assignments
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
PDB | |
DBJ | BAF83092 BAG59594 |
GB | AAB62226 AAC51300 AAF62400 AAI17450 AAI43646 |
REF | NP_001230022 NP_001456 NP_955367 XP_003274413 XP_003274414 |
SP | O15117 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts