BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5493

Title: Letter to the Editor: Backbone 1H, 15N and 13C resonance assignments of the Staphylococcus aureus acyl carrier protein synthase (AcpS)

Deposition date: 2002-08-07 Original release date: 2002-12-23

Authors: Liu, Dingjiang; Black, Todd; Macinga, David; Palermo, Robert; Wyss, Daniel

Citation: Liu, Dingjiang; Black, Todd; Macinga, David; Palermo, Robert; Wyss, Daniel. "Letter to the Editor: Backbone 1H, 15N and 13C resonance assignments of the Staphylococcus aureus acyl carrier protein synthase (AcpS)"  J. Biomol. NMR 24, 273-274 (2002).

Assembly members:
ACP synthase, polymer, 119 residues, Formula weight is not available

Natural source:   Common Name: Staphylococcus aureus   Taxonomy ID: 1280   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
ACP synthase: MIHGIGVDLIEIDRIQALYS KQPKLVERILTKNEQHKFNN FTHEQRKIEFLAGRFATKEA FSKALGTGLGKHVAFNDIDC YNDELGKPKIDYEGFIVHVS ISHTEHYAMSQVVLEKSAF

Data sets:
Data typeCount
13C chemical shifts312
15N chemical shifts106
1H chemical shifts106

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AcpS, unit I1
2AcpS, unit II1
3AcpS, unit III1

Entities:

Entity 1, AcpS, unit I 119 residues - Formula weight is not available

1   METILEHISGLYILEGLYVALASPLEUILE
2   GLUILEASPARGILEGLNALALEUTYRSER
3   LYSGLNPROLYSLEUVALGLUARGILELEU
4   THRLYSASNGLUGLNHISLYSPHEASNASN
5   PHETHRHISGLUGLNARGLYSILEGLUPHE
6   LEUALAGLYARGPHEALATHRLYSGLUALA
7   PHESERLYSALALEUGLYTHRGLYLEUGLY
8   LYSHISVALALAPHEASNASPILEASPCYS
9   TYRASNASPGLULEUGLYLYSPROLYSILE
10   ASPTYRGLUGLYPHEILEVALHISVALSER
11   ILESERHISTHRGLUHISTYRALAMETSER
12   GLNVALVALLEUGLULYSSERALAPHE

Samples:

sample_1: ACP synthase, [U-13C; U-15N; U-2H], 1.0 mM; KiPO4 0.075 M

condition_1: ionic strength: 0.45 M; pH: 7.5 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCOnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCACBnot availablenot availablenot available
1H-15N NOESY-HSQCnot availablenot availablenot available

Software:

FELIX v2000 - data processing

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAB43158 BAB58233 BAB95860 BAF68248 BAF78939
EMBL CAA76220 CAG41140 CAG43783 CAI81645 CAQ50498
GB AAW37023 ABD20944 ABD31340 ABQ49890 ABR52975
REF NP_372595 NP_375179 NP_646812 WP_000581193 WP_000581194
SP A5IUL7 A6QIR6 A6U3F7 A7X4P8 A8Z4X4

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts