BMRB Entry 5517

Name: NMR studies of the DNA-binding domain of B-Myb
Published: 2003-06-12

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PDB ID: 1a5j
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5517
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: NMR studies of the DNA-binding domain of B-Myb PubMed: 12815265

Deposition date: 2002-09-11 Original release date: 2003-06-12

Authors: Jones, Gareth

Citation: Jones, Gareth; Howard, Mark; McIntosh, Pauline; Williamson, Richard; Carr, Mark. "Sequence-specific Assignment of the B-Myb DNA-binding Domain (B-MybR2R3) bound to a 16 Base-pair DNA Target Site Corresponding to a Regulatory Site from the tom-1 Gene " J. Biomol. NMR 26, 375-376 (2003).

Assembly members:
B-MybR2R3-tom1a, polymer, 110 residues, 13095 Da.
tom1a, polymer, 16 residues, 4879 Da.
tom1a, polymer, 16 residues, 4879 Da.

Natural source: Common Name: Chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):
B-MybR2R3-tom1a: GIPDLVKGPWTKEEDQKVIE LVKKYGTKQWTLIAKHLKGR LGKQCRERWHNHLNPEVKKS SWTEEEDRIIFEAHKVLGNR WAEIAKLLPGRTDNAVKNHW NSTIKRKVDT
tom1a: CTCAGTCCGTTAAGGA
tom1a: TCCTTAACGGACTGAG

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	625
13C chemical shifts	289
15N chemical shifts	101

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Entity Assembly ID	Entity Name	Entity ID
1	B-MybR2R3 unit 1	1
2	tom1a 16mer	2
3	tom1a 16mer	3

1	GLY	ILE	PRO	ASP	LEU	VAL	LYS	GLY	PRO	TRP
2	THR	LYS	GLU	GLU	ASP	GLN	LYS	VAL	ILE	GLU
3	LEU	VAL	LYS	LYS	TYR	GLY	THR	LYS	GLN	TRP
4	THR	LEU	ILE	ALA	LYS	HIS	LEU	LYS	GLY	ARG
5	LEU	GLY	LYS	GLN	CYS	ARG	GLU	ARG	TRP	HIS
6	ASN	HIS	LEU	ASN	PRO	GLU	VAL	LYS	LYS	SER
7	SER	TRP	THR	GLU	GLU	GLU	ASP	ARG	ILE	ILE
8	PHE	GLU	ALA	HIS	LYS	VAL	LEU	GLY	ASN	ARG
9	TRP	ALA	GLU	ILE	ALA	LYS	LEU	LEU	PRO	GLY
10	ARG	THR	ASP	ASN	ALA	VAL	LYS	ASN	HIS	TRP
11	ASN	SER	THR	ILE	LYS	ARG	LYS	VAL	ASP	THR

Name	Sample	Sample state	Sample conditions
1H-15N NOESY	sample_1	not available	Ex-cond_1
HNCA	sample_1	not available	Ex-cond_1
HN(CO)CA	sample_1	not available	Ex-cond_1
CBCA(CO)NH	sample_1	not available	Ex-cond_1
HCCH-TOCSY	sample_1	not available	Ex-cond_1
1H-15N HSQC	sample_1	not available	Ex-cond_1

BMRB	4114
PDB	1A5J
DBJ	BAC25979 BAG37322 BAH13925 BAI46683
EMBL	CAA31655 CAA47839 CAA49898
GB	AAH07585 AAH50842 AAH53555 AAI18124 AAP36828
REF	NP_001068916 NP_001100006 NP_001231398 NP_001252784 NP_001265539
SP	P10244 P48972 Q03237
TPG	DAA23198

Biological Magnetic Resonance Data Bank

BMRB Entry 5517

Title: NMR studies of the DNA-binding domain of B-Myb PubMed: 12815265

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	GLY	ILE	PRO	ASP	LEU	VAL	LYS	GLY	PRO	TRP
2	THR	LYS	GLU	GLU	ASP	GLN	LYS	VAL	ILE	GLU
3	LEU	VAL	LYS	LYS	TYR	GLY	THR	LYS	GLN	TRP
4	THR	LEU	ILE	ALA	LYS	HIS	LEU	LYS	GLY	ARG
5	LEU	GLY	LYS	GLN	CYS	ARG	GLU	ARG	TRP	HIS
6	ASN	HIS	LEU	ASN	PRO	GLU	VAL	LYS	LYS	SER
7	SER	TRP	THR	GLU	GLU	GLU	ASP	ARG	ILE	ILE
8	PHE	GLU	ALA	HIS	LYS	VAL	LEU	GLY	ASN	ARG
9	TRP	ALA	GLU	ILE	ALA	LYS	LEU	LEU	PRO	GLY
10	ARG	THR	ASP	ASN	ALA	VAL	LYS	ASN	HIS	TRP
11	ASN	SER	THR	ILE	LYS	ARG	LYS	VAL	ASP	THR

1	GLY	ILE	PRO	ASP	LEU	VAL	LYS	GLY	PRO	TRP
2	THR	LYS	GLU	GLU	ASP	GLN	LYS	VAL	ILE	GLU
3	LEU	VAL	LYS	LYS	TYR	GLY	THR	LYS	GLN	TRP
4	THR	LEU	ILE	ALA	LYS	HIS	LEU	LYS	GLY	ARG
5	LEU	GLY	LYS	GLN	CYS	ARG	GLU	ARG	TRP	HIS
6	ASN	HIS	LEU	ASN	PRO	GLU	VAL	LYS	LYS	SER
7	SER	TRP	THR	GLU	GLU	GLU	ASP	ARG	ILE	ILE
8	PHE	GLU	ALA	HIS	LYS	VAL	LEU	GLY	ASN	ARG
9	TRP	ALA	GLU	ILE	ALA	LYS	LEU	LEU	PRO	GLY
10	ARG	THR	ASP	ASN	ALA	VAL	LYS	ASN	HIS	TRP
11	ASN	SER	THR	ILE	LYS	ARG	LYS	VAL	ASP	THR

1	GLY	ILE	PRO	ASP	LEU	VAL	LYS	GLY	PRO	TRP
2	THR	LYS	GLU	GLU	ASP	GLN	LYS	VAL	ILE	GLU
3	LEU	VAL	LYS	LYS	TYR	GLY	THR	LYS	GLN	TRP
4	THR	LEU	ILE	ALA	LYS	HIS	LEU	LYS	GLY	ARG
5	LEU	GLY	LYS	GLN	CYS	ARG	GLU	ARG	TRP	HIS
6	ASN	HIS	LEU	ASN	PRO	GLU	VAL	LYS	LYS	SER
7	SER	TRP	THR	GLU	GLU	GLU	ASP	ARG	ILE	ILE
8	PHE	GLU	ALA	HIS	LYS	VAL	LEU	GLY	ASN	ARG
9	TRP	ALA	GLU	ILE	ALA	LYS	LEU	LEU	PRO	GLY
10	ARG	THR	ASP	ASN	ALA	VAL	LYS	ASN	HIS	TRP
11	ASN	SER	THR	ILE	LYS	ARG	LYS	VAL	ASP	THR