BMRB Entry 5594
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5594
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Title: Structural insights into the U-box, a domain associated with multi-ubiquitination PubMed: 12627222
Deposition date: 2002-11-21 Original release date: 2003-04-22
Authors: Ohi, Melanie; Vander Kooi, Craig; Rosenberg, Joshua; Chazin, Walter; Gould, Kathleen
Citation: Ohi, Melanie; Vander Kooi, Craig; Rosenberg, Joshua; Chazin, Walter; Gould, Kathleen. "Structural Insights into the U-box, a Domain Associated with Multi-ubiquitination" Nat. Struct. Biol. 10, 250-255 (2003).
Assembly members:
Prp19, polymer, 52 residues, Formula weight is not available
Natural source: Common Name: Baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Prp19: MLCAISGKVPRRPVLSPKSR
TIFEKSLLEQYVKDTGNDPI
TNEPLSIEEIVE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 141 |
| 15N chemical shifts | 46 |
| 1H chemical shifts | 313 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Prp19(1-73), ubox | 1 |
Entities:
Entity 1, Prp19(1-73), ubox 52 residues - Formula weight is not available
| 1 | MET | LEU | CYS | ALA | ILE | SER | GLY | LYS | VAL | PRO | ||||
| 2 | ARG | ARG | PRO | VAL | LEU | SER | PRO | LYS | SER | ARG | ||||
| 3 | THR | ILE | PHE | GLU | LYS | SER | LEU | LEU | GLU | GLN | ||||
| 4 | TYR | VAL | LYS | ASP | THR | GLY | ASN | ASP | PRO | ILE | ||||
| 5 | THR | ASN | GLU | PRO | LEU | SER | ILE | GLU | GLU | ILE | ||||
| 6 | VAL | GLU |
Samples:
sample_1: Prp19, [U-13C; U-15N], 1.0 mM; sodium phosphate 20 mM; NaCl 20 mM
sample_2: Prp19, [U-15N], 0.75 mM; sodium phosphate 20 mM; NaCl 20 mM
sample_3: Prp19 1.0 mM; sodium phosphate 20 mM; NaCl 20 mM
Cond_1: ionic strength: 0.28 M; pH: 7.0; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCA | not available | not available | Cond_1 |
| HN(CO)CA | not available | not available | Cond_1 |
| HNCACB | not available | not available | Cond_1 |
| CBCA(CO)NH | not available | not available | Cond_1 |
| 1H-1H NOE | not available | not available | Cond_1 |
| 1H-1H COSY | not available | not available | Cond_1 |
| 1H-1H TOCSY | not available | not available | Cond_1 |
| 1H-15N HSQC | not available | not available | Cond_1 |
| 1H-1H-15N NOE | not available | not available | Cond_1 |
| 1H-1H-15N TOCSY | not available | not available | Cond_1 |
| 1H-1H-13C NOE | not available | not available | Cond_1 |
| 1H-1H-13C TOCSY | not available | not available | Cond_1 |
Software:
xwinnmr v2.6 - data processing
XEASY v1.3.13 - assignment, peak integration
DYANA v1.5 - calibration, structure calculation
AMBER v6 - restrained molecular dynamics
NMR spectrometers:
- Bruker Avance 500 MHz
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
Related Database Links:
| PDB | |
| DBJ | GAA24853 |
| EMBL | CAA68103 CAA97487 CAY81203 |
| GB | AAA34912 AAM90948 AHY78374 EDN59512 EDV09279 |
| REF | NP_013064 |
| SP | P32523 |
| TPG | DAA09285 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts