BMRB Entry 5656
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5656
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Title: Resonance Assignments for the Z Domain of Staphylococcal Protein A using a 2H, 13C, 15N enriched, selective methyl protonated sample PubMed: 14718654
Deposition date: 2003-01-09 Original release date: 2005-05-03
Authors: Zheng, Deyou; Swapna, G.V.T.; Hunter, Moseley; Montelione, Gaetano
Citation: Zheng, D.; Aramini, J.; Montelione, G.. "Validation of helical tilt angles in the solution NMR structure of the Z domain of Staphylococcal protein A by combined analysis of residual dipolar coupling and NOE data." Protein Sci. 13, 549-554 (2004).
Assembly members:
staphylococcal protein A Z domain, polymer, 71 residues, Formula weight is not available
Natural source: Common Name: S. aureus Taxonomy ID: 1280 Superkingdom: Eubacteria Kingdom: not available Genus/species: Staphylococcus aureus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
staphylococcal protein A Z domain: KAIFVLNAQHDEAVDNKFNK
EQQNAFYEILHLPNLNEEQR
NAFIQSLKDDPSQSANLLAE
AKKLNDAQAPK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 168 |
| 13C chemical shifts | 235 |
| 15N chemical shifts | 83 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | staphylococcal protein A Z domain | 1 |
Entities:
Entity 1, staphylococcal protein A Z domain 71 residues - Formula weight is not available
| 1 | LYS | ALA | ILE | PHE | VAL | LEU | ASN | ALA | GLN | HIS | ||||
| 2 | ASP | GLU | ALA | VAL | ASP | ASN | LYS | PHE | ASN | LYS | ||||
| 3 | GLU | GLN | GLN | ASN | ALA | PHE | TYR | GLU | ILE | LEU | ||||
| 4 | HIS | LEU | PRO | ASN | LEU | ASN | GLU | GLU | GLN | ARG | ||||
| 5 | ASN | ALA | PHE | ILE | GLN | SER | LEU | LYS | ASP | ASP | ||||
| 6 | PRO | SER | GLN | SER | ALA | ASN | LEU | LEU | ALA | GLU | ||||
| 7 | ALA | LYS | LYS | LEU | ASN | ASP | ALA | GLN | ALA | PRO | ||||
| 8 | LYS |
Samples:
sample_one: staphylococcal protein A Z domain, [U-13C; U-15N; U-2H] with Val, Leu, Ile(delta) methyl protonated, 1.1 mM
sample_conditions_one: pH: 6.5; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| 1H-13C HSQC | not available | not available | not available |
| HNCO | not available | not available | not available |
| HN(CA)CO | not available | not available | not available |
| HNCACB | not available | not available | not available |
| HN(CO)CACB | not available | not available | not available |
| H(CCCO)NH-TOCSY | not available | not available | not available |
| (H)CC(CO)NH-TOCSY | not available | not available | not available |
Software:
AutoAssign v1.9 - automated backbone assignments
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| BMRB | 19043 4023 |
| PDB | |
| DBJ | BAC76617 BAC76619 BAL27690 BAL27694 BAL27697 |
| EMBL | CAA49867 CAA65431 |
| GB | AAA72944 AAB00807 AAR10383 AAR10384 AAR10385 |
| REF | WP_000298178 WP_000298179 WP_000316486 WP_000363686 WP_000496862 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts