BMRB Entry 5689
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5689
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Title: 1H, 13C, and 15N backbone assignments of the pheromone binding protein from the silk moth Antheraea polyphemus (ApolPBP) PubMed: 14512737
Deposition date: 2003-02-07 Original release date: 2005-10-17
Authors: Mohanty, Smita; Zubkov, Sergey; Campos-Olivas, Ramon
Citation: Mohanty, Smita; Zubkov, Sergey; Campos-Olivas, Ramon. "Letter to the Editor: 1H, 13C and 15N Backbone Assignments of the Pheromone Binding Protein from the Silk Moth Antheraea polyphemus (ApolPBP)" J. Biomol. NMR 27, 393-394 (2003).
Assembly members:
Pheromone-binding protein from Antheraea polyphemus, polymer, 142 residues, Formula weight is not available
Natural source: Common Name: Polyphemus moth Taxonomy ID: 7120 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Antheraea polyphemus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Pheromone-binding protein from Antheraea polyphemus: SPEIMKNLSNNFGKAMDQCK
DELSLPDSVVADLYNFWKDD
YVMTDRLAGCAINCLATKLD
VVDPDGNLHHGNAKDFAMKH
GADETMAQQLVDIIHGCEKS
APPNDDKCMKTIDVAMCFKK
EIHKLNWVPNMDLVIGEVLA
EV
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 548 |
| 15N chemical shifts | 147 |
| 1H chemical shifts | 939 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ApolPBP | 1 |
Entities:
Entity 1, ApolPBP 142 residues - Formula weight is not available
| 1 | SER | PRO | GLU | ILE | MET | LYS | ASN | LEU | SER | ASN | ||||
| 2 | ASN | PHE | GLY | LYS | ALA | MET | ASP | GLN | CYS | LYS | ||||
| 3 | ASP | GLU | LEU | SER | LEU | PRO | ASP | SER | VAL | VAL | ||||
| 4 | ALA | ASP | LEU | TYR | ASN | PHE | TRP | LYS | ASP | ASP | ||||
| 5 | TYR | VAL | MET | THR | ASP | ARG | LEU | ALA | GLY | CYS | ||||
| 6 | ALA | ILE | ASN | CYS | LEU | ALA | THR | LYS | LEU | ASP | ||||
| 7 | VAL | VAL | ASP | PRO | ASP | GLY | ASN | LEU | HIS | HIS | ||||
| 8 | GLY | ASN | ALA | LYS | ASP | PHE | ALA | MET | LYS | HIS | ||||
| 9 | GLY | ALA | ASP | GLU | THR | MET | ALA | GLN | GLN | LEU | ||||
| 10 | VAL | ASP | ILE | ILE | HIS | GLY | CYS | GLU | LYS | SER | ||||
| 11 | ALA | PRO | PRO | ASN | ASP | ASP | LYS | CYS | MET | LYS | ||||
| 12 | THR | ILE | ASP | VAL | ALA | MET | CYS | PHE | LYS | LYS | ||||
| 13 | GLU | ILE | HIS | LYS | LEU | ASN | TRP | VAL | PRO | ASN | ||||
| 14 | MET | ASP | LEU | VAL | ILE | GLY | GLU | VAL | LEU | ALA | ||||
| 15 | GLU | VAL |
Samples:
sample_1: Pheromone-binding protein from Antheraea polyphemus, [U-99% 13C; U-99% 15N], 1.0 mM
backbone_conditions: pH: 6.33; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | not available | not available | not available |
| 2D 1H-13C HMQC | not available | not available | not available |
| 3D HNCA | not available | not available | not available |
| 3D CBCA(CO)NH | not available | not available | not available |
| 3D HNCO | not available | not available | not available |
| 3D CC(CO)NH | not available | not available | not available |
| 3D HCACO | not available | not available | not available |
| 3D HNCACB | not available | not available | not available |
| 3D 1H-15N-1H NOESY | not available | not available | not available |
| 3D 1H-13C-1H NOESY | not available | not available | not available |
Software:
NMRPipe v2.1 -
NMRView v5.0.4 -
NMR spectrometers:
- Bruker DMX 500 MHz
- Bruker DMX 600 MHz
- Bruker DRX 800 MHz
Related Database Links:
| BMRB | 15256 |
| PDB | |
| EMBL | CAA35592 |
| GenBank | AAB49502 |
| PRF | 1515253A |
| SWISS-PROT | P20797 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts