BMRB Entry 5708
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5708
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Title: Solution structure of the N-terminal membrane anchor of E. coli enzyme IIA(Glucose) PubMed: 12717030
Deposition date: 2003-02-25 Original release date: 2003-05-22
Authors: Wang, G.; Keifer, P.; Peterkofsky, A.
Citation: Wang, G.; Keifer, P.; Peterkofsky, A.. "Solution Structure of the N-terminal Amphitropic Domain of Escherichia coli Glucose-specific Enzyme IIA in Membrane-mimetic Micelles" Protein Sci. 12, 1087-1096 (2003).
Assembly members:
PTS, polymer, 15 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eukaryota Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
PTS: GLFDKLKSLVSDDKK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 191 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PTS system, glucose-specific IIA component | 1 |
Entities:
Entity 1, PTS system, glucose-specific IIA component 15 residues - Formula weight is not available
| 1 | GLY | LEU | PHE | ASP | LYS | LEU | LYS | SER | LEU | VAL | ||||
| 2 | SER | ASP | ASP | LYS | LYS |
Samples:
sample_1: PTS 5 mM; H2O 90%; D2O 10%; SDS 100 mM
sample_2: PTS 5 mM; DHPG 50 mM
sample_cond_1: pH: 5.4; temperature: 298 K; ionic strength: 0 M; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | not available | not available | not available |
| TOCSY | not available | not available | not available |
| DQF-COSY | not available | not available | not available |
Software:
NMRPipe v2.1 - data processing
nmrDraw v2.1 - data processing
PIPP v1.0 - NOE picking
XPLOR-NIH v1.06 - structural calculations
MOLMOL v2K.1 - structural analysis, structural viewing
NMR spectrometers:
- Varian INOVA 600 MHz