BMRB Entry 5892
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5892
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Title: 1H, 13C and 15N NMR Assignments of the Hyperthermophile Sulfolobus Protein Sso10b2 and its Predicted Secondary Structure PubMed: 16245938
Deposition date: 2003-07-30 Original release date: 2007-11-05
Authors: Biyani, Kalpesh; Kahsai, Mebrahtu; Edmondson, Stephen; Shriver, John
Citation: Biyani, Kalpesh; Kahsai, Mebrahtu; Clark, Andrew; Armstrong, Tracy; Edmondson, Stephen; Shriver, John. "Solution structure, stability, and nucleic acid binding of the hyperthermophile protein Sso10b2" Biochemistry 44, 14217-14230 (2005).
Assembly members:
Sso10b2, polymer, 89 residues, Formula weight is not available
Natural source: Common Name: Sulfolobus solfataricus Taxonomy ID: 2287 Superkingdom: Archaea Kingdom: not available Genus/species: Sulfolobus solfataricus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Sso10b2: MTEKLNEIVVRKTKNVEDHV
LDVIVLFNQGIDEVILKGTG
REISKAVDVYNSLKDRLGDG
VQLVNVQTGSEVRDRRRISY
ILLRLKRVY
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 617 |
| 13C chemical shifts | 400 |
| 15N chemical shifts | 87 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Sso10b2 subunit 1 | 1 |
| 2 | Sso10b2 subunit 2 | 1 |
Entities:
Entity 1, Sso10b2 subunit 1 89 residues - Formula weight is not available
| 1 | MET | THR | GLU | LYS | LEU | ASN | GLU | ILE | VAL | VAL | ||||
| 2 | ARG | LYS | THR | LYS | ASN | VAL | GLU | ASP | HIS | VAL | ||||
| 3 | LEU | ASP | VAL | ILE | VAL | LEU | PHE | ASN | GLN | GLY | ||||
| 4 | ILE | ASP | GLU | VAL | ILE | LEU | LYS | GLY | THR | GLY | ||||
| 5 | ARG | GLU | ILE | SER | LYS | ALA | VAL | ASP | VAL | TYR | ||||
| 6 | ASN | SER | LEU | LYS | ASP | ARG | LEU | GLY | ASP | GLY | ||||
| 7 | VAL | GLN | LEU | VAL | ASN | VAL | GLN | THR | GLY | SER | ||||
| 8 | GLU | VAL | ARG | ASP | ARG | ARG | ARG | ILE | SER | TYR | ||||
| 9 | ILE | LEU | LEU | ARG | LEU | LYS | ARG | VAL | TYR |
Samples:
sample_1: Sso10b2, [U-98% 13C; U-98% 15N], 1.0 mM
Condition_1: pH: 4.6; temperature: 303 K; ionic strength: 0.0 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
Varian -
NMRView -
NMRPipe -
Felix -
NMR spectrometers:
- VARIAN INOVA 800 MHz
Related Database Links:
| PDB | |
| EMBL | CAA67067 |
| GB | AAK41238 ACP35355 ACP38015 ACP45511 ACP48691 |
| REF | NP_342448 WP_009992410 WP_012711268 YP_002829313 YP_002832000 |
| SP | P74762 Q97ZF4 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts