BMRB Entry 5945
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5945
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Title: A Zinc Clasp Structure Tethers Lck to T Cell Coreceptors CD4 and CD8 PubMed: 14500983
Deposition date: 2003-09-11 Original release date: 2003-12-19
Authors: Kim, Peter; Sun, Zhen-Yu; Blacklow, Stephen; Wagner, Gerhard; Eck, Michael
Citation: Kim, Peter; Sun, Zhen-Yu; Blacklow, Stephen; Wagner, Gerhard; Eck, Michael. "A Zinc Clasp Structure Tethers Lck to T Cell Coreceptors CD4 and CD8" Science (Washington, DC, U. S.) 301, 1725-1728 (2003).
Assembly members:
CD8a, polymer, 19 residues, Formula weight is not available
Lck, polymer, 29 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
CD8a: RNRRRVCKCPRPVVKSGDK
Lck: SHPEDDWLENIDVCENCHYP
IVPLDGKGT
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 268 |
| 13C chemical shifts | 148 |
| 15N chemical shifts | 41 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CD8a cytoplasmic tail | 1 |
| 2 | Lck N-terminus | 2 |
| 3 | ZINC (II) ION | 3 |
Entities:
Entity 1, CD8a cytoplasmic tail 19 residues - Formula weight is not available
| 1 | ARG | ASN | ARG | ARG | ARG | VAL | CYS | LYS | CYS | PRO | ||||
| 2 | ARG | PRO | VAL | VAL | LYS | SER | GLY | ASP | LYS |
Entity 2, Lck N-terminus 29 residues - Formula weight is not available
| 1 | SER | HIS | PRO | GLU | ASP | ASP | TRP | LEU | GLU | ASN | ||||
| 2 | ILE | ASP | VAL | CYS | GLU | ASN | CYS | HIS | TYR | PRO | ||||
| 3 | ILE | VAL | PRO | LEU | ASP | GLY | LYS | GLY | THR |
Entity 3, ZINC (II) ION - Zn - 65.409 Da.
| 1 | ZN |
Samples:
Sample_1: CD8a 0.5 mM; Lck 0.5 mM
Condition_set_1: pH: 5.1; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCA | Sample_1 | not available | Condition_set_1 |
| HN(CO)CA | Sample_1 | not available | Condition_set_1 |
| HNCACB | Sample_1 | not available | Condition_set_1 |
| HN(CO)CACB | Sample_1 | not available | Condition_set_1 |
| CCONH | Sample_1 | not available | Condition_set_1 |
| HCCONH | Sample_1 | not available | Condition_set_1 |
| HCCH TOCSY | Sample_1 | not available | Condition_set_1 |
| 15N-NOESY | Sample_1 | not available | Condition_set_1 |
| 15N-TOCSY | Sample_1 | not available | Condition_set_1 |
| D2O NOESY | Sample_1 | not available | Condition_set_1 |
| D2O TOCSY | Sample_1 | not available | Condition_set_1 |
| Cd-H HMQC | Sample_1 | not available | Condition_set_1 |
| Cd-H NOESY-HMQC | Sample_1 | not available | Condition_set_1 |
Software:
FELIX - processing
DYANA - structure solution
X-PLOR - structure solution
NMR spectrometers:
- Bruker Varian 500 MHz
- Bruker Varian 750 MHz
Related Database Links:
| SWISS-PROT | P01732 |
| REF | XP_001138871 XP_001138772 NP_741969 NP_001759 |
| GenBank | AAB04637 AAA79218 AAA79217 AAA61133 AAA59674 |
| DBJ | BAG61892 BAG53555 |
| PDB | |
| BMRB | 5944 |
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