BMRB Entry 5981
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PDB ID: 1yho
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5981
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Title: 1H, 15N and 13C resonance assignments of human dihydrofolate reductase in its complex with trimethoprim and NADPH PubMed: 16222560
Deposition date: 2003-10-23 Original release date: 2006-01-19
Authors: Kovalevskaya, Nadezhda; Polshakov, Vladimir; Birdsall, Berry; Bradbury, Alan; Frenkiel, Thomas; Feeney, James
Citation: Kovalevskaya, Nadezhda; Smurnyy, Yegor; Polshakov, Vladimir; Birdsall, Berry; Bradbury, Alan; Frenkiel, Thomas; Feeney, James. "Solution structure of human dihydrofolate reductase in its complex with trimethoprim and NADPH " J. Biomol. NMR 33, 69-72 (2005).
Assembly members:
Dihydrofolate reductase, polymer, 186 residues, 21300 Da.
NAP, non-polymer, 743.405 Da.
TOP, non-polymer, 290.318 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Dihydrofolate reductase: VGSLNCIVAVSQNMGIGKNG
DLPWPPLRNEFRYFQRMTTT
SSVEGKQNLVIMGKKTWFSI
PEKNRPLKGRINLVLSRELK
EPPQGAHFLSRSLDDALKLT
EQPELANKVDMVWIVGGSSV
YKEAMNHPGHLKLFVTRIMQ
DFESDTFFPEIDLEKYKLLP
EYPGVLSDVQEEKGIKYKFE
VYEKND
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 1146 |
| 13C chemical shifts | 706 |
| 15N chemical shifts | 204 |
Additional metadata:
Related Database Links:
| BMRB | 17096 19563 19564 19565 19566 19567 7195 |
| PDB | 1BOZ 1DHF 1DLR 1DLS 1DRF 1HFP 1HFQ 1HFR 1KMS 1KMV 1MVS 1MVT 1OHJ 1OHK 1PD8 1PD9 1PDB 1S3U 1S3V 1S3W 1U71 1U72 1YHO 2C2S 2C2T 2DHF 2W3A 2W3B 2W3M 3EIG 3F8Y 3F8Z 3F91 3FS6 3GHC 3GHV 3GHW 3GI2 3GYF 3L3R 3N0H 3NTZ 3NU0 3NXO 3NXR 3NXT 3NXV 3NXX 3NXY 3NZD 3OAF 3S3V 3S7A 4DDR 4G95 4KAK 4KBN 4KD7 4KEB 4KFJ 4M6J 4M6K 4M6L |
| DBJ | BAG35526 BAG56693 BAG59770 BAJ20267 |
| EMBL | CAA23765 CAA25409 |
| GB | AAA58484 AAA58485 AAH00192 AAH03584 AAH70280 |
| PRF | 0905195A 0906214A |
| REF | NP_000782 NP_001277283 NP_001277286 XP_001099963 XP_001110551 |
| SP | P00374 |
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