BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6087

Title: Conformation and Ion-Channeling activity of the hepatitis C virus p7 polypeptide   PubMed: 20667830

Deposition date: 2004-01-29 Original release date: 2005-01-20

Authors: Montserret, Roland; Saint, Nathalie; Simorre, Jean Pierre; Penin, Francois

Citation: Montserret, Roland; Saint, Nathalie; Vanbelle, Christophe; Salvay, Andres; Simorre, Jean-Pierre; Ebel, Christine; Sapay, Nicolas; Renisio, Jean-Guillaume; Bockmann, Anja; Steinmann, Eike; Pietschmann, Thomas; Dubuisson, Jean; Chipot, Christophe; Penin, Francois. "NMR structure and ion channel activity of the p7 protein from hepatitis C virus"  J. Biol. Chem. ., .-. (2010).

Assembly members:
p7, polymer, 63 residues, 6698.16 Da.

Natural source:   Common Name: HCV   Taxonomy ID: 11103   Superkingdom: Viruses   Kingdom: not available   Genus/species: Hepacivirus Hepatitis C virus

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
p7: TLENLVVLNAASVAGAHGLL SFLVFFAAAWYIKGRLVPGA AYALYGVWPLLLLLLALPPR AYA

Data sets:
Data typeCount
1H chemical shifts472
13C chemical shifts167

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1HCV p71

Entities:

Entity 1, HCV p7 63 residues - 6698.16 Da.

1   THRLEUGLUASNLEUVALVALLEUASNALA
2   ALASERVALALAGLYALAHISGLYLEULEU
3   SERPHELEUVALPHEPHEALAALAALATRP
4   TYRILELYSGLYARGLEUVALPROGLYALA
5   ALATYRALALEUTYRGLYVALTRPPROLEU
6   LEULEULEULEULEUALALEUPROPROARG
7   ALATYRALA

Samples:

sample_1: p7 1.26 mM; 2,2,2-trifluoroethanol-d2 90%; H2O 10%

condition_1: pH: 3.0; temperature: 301 K

Experiments:

NameSampleSample stateSample conditions
NOESYnot availablenot availablenot available
TOCSYnot availablenot availablenot available
1H-13C-HSQCnot availablenot availablenot available
HSQC-TOCSY in 13C natural abondancy.not availablenot availablenot available

Software:

vnmr v6.1 - acquisition, processing

Sparky v3.106 - Analysis

NMR spectrometers:

  • Varian Unity-plus 500 MHz
  • Varian Inova 800 MHz

Related Database Links:

DBJ BAA03905 BAA05974 BAA05975 BAA14035 BAA14233
EMBL CAC81084 CAC81085 CAC81086 CAC81087 CAC81088
GB AAK08509 ABV46113 ACA50684 ACT67346 ADV92207
SP P26662