BMRB Entry 6239

Name: Chemical shifts assignments for stem-loop VI of the VS ribozyme.
Published: 2004-09-20

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6239

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Chemical shifts assignments for stem-loop VI of the VS ribozyme. PubMed: 15328609

Deposition date: 2004-06-15 Original release date: 2004-09-20

Authors: Dieckmann, Thorsten; Flinders, Jeremy

Citation: Flinders, Jeremy; Dieckmann, Thorsten. "The Solution Structure of the VS Ribozyme Active Site Loop Reveals a Dynamic 'Hotspot'" J. Mol. Biol. 341, 935-949 (2004).

Assembly members:
VSVI RNA, polymer, 22 residues, Formula weight is not available

Natural source: Common Name: Neurospora crassa Taxonomy ID: 5141 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Neurospora crassa

Experimental source: Production method: enzymatic semisynthesis

Entity Sequences (FASTA):
VSVI RNA: GGUGACGCCGUAAGGCGCAG CC

Data sets:

assigned_chemical_shifts
- shift_1

Data type	Count
13C chemical shifts	139
15N chemical shifts	1
1H chemical shifts	183

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	VSVI RNA	1

Entities:

Entity 1, VSVI RNA 22 residues - Formula weight is not available

1

G

U

G

A

C

G

C

G

2

U

A

G

C

G

C

A

G

3

C

Samples:

sample_1: VSVI RNA 1.5 mM

sample_2: VSVI RNA, [U-95% 13C; U-95% 15N], 1.2 mM

sample_3: VSVI RNA, [U-95% 15N], 1.5 mM

Ex-cond_1: pH: 6.0; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
not available	not available	not available	Ex-cond_1

Software:

XWIN-NMR v3.5, Bruker - Acquisition, Processing

SPARKY v3.106, UCSF - Analysis (Peak Picking)

NMR spectrometers:

Bruker DRX 600 MHz