BMRB Entry 6273
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6273
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Title: 1H and 31P chemical shift assignments for the triloop DNA hairpin 5'- GTTCACAGAAC - 3' PubMed: 16530466
Deposition date: 2004-07-23 Original release date: 2004-12-14
Authors: Patard, Louis; Cordier, Christine
Citation: Lamoureux, Michele; Patard, Louis; Hernandez, Belen; Couesnon, Thierry; Santini, Guillaume; Cognet, Jean; Gouyette, Catherine; Cordier, Christine. "Spectroscopic and structural impact of a stem base-pair change in DNA hairpins: GTTC-ACA-GAAC versus GTAC-ACA-GTAC." Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 65, 84-94 (2006).
Assembly members:
DNA hairpin, polymer, 11 residues, 3493 Da.
Natural source: Common Name: Vibrio cholerae Taxonomy ID: 666 Superkingdom: Eubacteria Kingdom: not available Genus/species: vibrio cholerae
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
DNA hairpin: GTTCACAGAAC
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 101 |
| 31P chemical shifts | 10 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | hairpin DNA | 1 |
Entities:
Entity 1, hairpin DNA 11 residues - 3493 Da.
| 1 | DG | DT | DT | DC | DA | DC | DA | DG | DA | DA | ||||
| 2 | DC |
Samples:
sample_1: DNA hairpin 6.8 mM; phosphate 20.6 mM; EDTA 1.0 mM; D2O 10 % v/v
sample_2: DNA hairpin 6.8 mM; phosphate 20.6 mM; EDTA 1.0 mM; D2O 99.9 % v/v
Ex-cond_1: ionic strength: 0.08 M; pH*: 7.2; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-1H NOESY | not available | not available | Ex-cond_1 |
| 1H-1H TOCSY | not available | not available | Ex-cond_1 |
| 1H-1H DQF-COSY | not available | not available | Ex-cond_1 |
| 1H-31P COLOC | not available | not available | Ex-cond_1 |
Software:
GIFA v4.2 - integration, Processing of the specta
NMR spectrometers:
- Bruker DRX 500 MHz