BMRB Entry 6320

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6320

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: 1H, 13C, 15N chemical shift assignments for extended 3' internal stem-loop RNA from S. cerevisiae U6 snRNA PubMed: 15543154

Deposition date: 2004-09-21 Original release date: 2004-11-16

Authors: Sashital, Dipali; Cornilescu, Gabriel; Butcher, Samuel

Citation: Sashital, Dipali; Cornilescu, Gabriel; McManus, C.; Brow, D.; Butcher, Samuel. "U2-U6 RNA folding reveals a group II intron-like domain and a four-helix junction" Nat. Struct. Mol. Biol. 11, 1237-1242 (2004).

Assembly members:
U6 extended ISL, polymer, 32 residues, Formula weight is not available

Natural source: Common Name: baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi

Experimental source: Production method: enzymatic semisynthesis

Entity Sequences (FASTA):
U6 extended ISL: GAGCAGUUCCCCUGCAUAAG GAUGAACCGUUC

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts

Data type	Count
13C chemical shifts	189
15N chemical shifts	14
1H chemical shifts	272

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	U6 extended ISL	1

Entities:

Entity 1, U6 extended ISL 32 residues - Formula weight is not available

1

G

A

G

C

A

G

U

C

2

C

U

G

C

A

U

A

G

3

G

A

U

G

A

C

G

U

4

U

C

Samples:

sample_1: U6 extended ISL, [U-13C; U-15N], 1 mM

sample_conditions_1: pH: 6.9; temperature: 283 K

sample_conditions_2: pH: 7.5; temperature: 303 K

sample_conditions_3: pH: 6.9; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D NOESY	sample_1	not available	not available
2D TOCSY	sample_1	not available	not available
3D [1H-13C-1H] NOESY HMQC	sample_1	not available	not available
3D [1H-13C-1H] HCCH-TOCSY	sample_1	not available	not available
3D [1H-13C-1H] HCCH-COSY	sample_1	not available	not available
J-Modulated CT-HSQC	sample_1	not available	not available

Software:

SPARKY v3.105 -

NMR spectrometers:

Bruker DMX 600 MHz
Varian Inova 600 MHz
Bruker DMX 750 MHz
Varian Inova 800 MHz