BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6433

Title: Sequence specific 1HN, 13C and 15N resonance assignments of a novel calcium binding protein from Entamoeba histolytica   PubMed: 15929013

Deposition date: 2004-12-16 Original release date: 2005-07-26

Authors: Bansal-Mutalik, Ritu; Mustafi, Sourajit; Bhattacharya, Alok; Chary, V. R.

Citation: Bansal-Mutalik, Ritu; Mustafi, Sourajit; Bhattacharya, Alok; Chary, Kandala. "Letter to the Editor: Sequence specific 1HN, 13C and 15N resonance assignments of a novel calcium-binding protein from Entamoeba histolytica."  J. Biomol. NMR 31, 379-380 (2005).

Assembly members:
Entamoeba histolytica calcium binding protein, polymer, 134 residues, 14944 Da.
CA, non-polymer, 40.078 Da.

Natural source:   Common Name: Escherichia coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Entamoeba histolytica calcium binding protein: MAEALFKQLDANGDGSVSYE EVKAFVSSKRPIKNEQLLQL IFKAIDIDGNGEIDLAEFTK FAAAVKEQDLSDEKVGLKIL YKLMDADGDGKLTKEEVTTF FKKFGYEKVVDQIMKADANG DGYITLEEFLAFNL

Data sets:
Data typeCount
13C chemical shifts373
15N chemical shifts123
1H chemical shifts577

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1EhCaBP21
2CALCIUM (II) ION2

Entities:

Entity 1, EhCaBP2 134 residues - 14944 Da.

1   METALAGLUALALEUPHELYSGLNLEUASP
2   ALAASNGLYASPGLYSERVALSERTYRGLU
3   GLUVALLYSALAPHEVALSERSERLYSARG
4   PROILELYSASNGLUGLNLEULEUGLNLEU
5   ILEPHELYSALAILEASPILEASPGLYASN
6   GLYGLUILEASPLEUALAGLUPHETHRLYS
7   PHEALAALAALAVALLYSGLUGLNASPLEU
8   SERASPGLULYSVALGLYLEULYSILELEU
9   TYRLYSLEUMETASPALAASPGLYASPGLY
10   LYSLEUTHRLYSGLUGLUVALTHRTHRPHE
11   PHELYSLYSPHEGLYTYRGLULYSVALVAL
12   ASPGLNILEMETLYSALAASPALAASNGLY
13   ASPGLYTYRILETHRLEUGLUGLUPHELEU
14   ALAPHEASNLEU

Entity 2, CALCIUM (II) ION - Ca - 40.078 Da.

1   CA

Samples:

sample_1: Entamoeba histolytica calcium binding protein, [U-13C; U-15N], 1.5 mM

cond_1: pH: 4.2; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
N15 HSQCsample_1not availablecond_1
CBCANHsample_1not availablecond_1
CBCA(CO)NHsample_1not availablecond_1
HNCAsample_1not availablecond_1
HN(CA)COsample_1not availablecond_1
HNCOsample_1not availablecond_1

Software:

Felix - Data processing

NMR spectrometers:

  • Varian . 600 MHz

Related Database Links:

PDB
DBJ BAA22013
GB AAR97975 EAL51694 EDR27146 EKE36904 EMD43571
REF XP_001736602 XP_008860769 XP_657080

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts