BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 644

Title: Proline Assignments and Identification of the Cis K116/P117 Peptide Bond in Liganded Staphylococcal Nuclease Using Isotope Edited 2D NMR Spectroscopy

Deposition date: 1995-07-31 Original release date: 1995-07-31

Authors: Torchia, Dennis; Sparks, Steven; Young, Paul; Bax, Ad

Citation: Torchia, Dennis; Sparks, Steven; Young, Paul; Bax, Ad. "Proline Assignments and Identification of the Cis K116/P117 Peptide Bond in Liganded Staphylococcal Nuclease Using Isotope Edited 2D NMR Spectroscopy"  J. Am. Chem. Soc. 111, 8315-8317 (1989).

Assembly members:
micrococcal nuclease, polymer, 156 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: 1280   Superkingdom: not available   Kingdom: Staphylococcus   Genus/species: aereus not available

Experimental source:   Production method: not available

Entity Sequences (FASTA):
micrococcal nuclease: MDPTVYSATSTKKLHKEPAT LIKAIDGDTVKLMYKGQPMT FRLLLVDTPETKHPKKGVEK YGPEASAFTKKMVENAKKIE VEFNKGQRTDKYGRGLAYIY ADGKMVNEALVRQGLAKVAY VYKPNNTHEQHLRKSEAQAK KEKLNIWSENDADSGQ

Data sets:
Data typeCount
13C chemical shifts12
1H chemical shifts42

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1micrococcal nuclease1

Entities:

Entity 1, micrococcal nuclease 156 residues - Formula weight is not available

1   METASPPROTHRVALTYRSERALATHRSER
2   THRLYSLYSLEUHISLYSGLUPROALATHR
3   LEUILELYSALAILEASPGLYASPTHRVAL
4   LYSLEUMETTYRLYSGLYGLNPROMETTHR
5   PHEARGLEULEULEUVALASPTHRPROGLU
6   THRLYSHISPROLYSLYSGLYVALGLULYS
7   TYRGLYPROGLUALASERALAPHETHRLYS
8   LYSMETVALGLUASNALALYSLYSILEGLU
9   VALGLUPHEASNLYSGLYGLNARGTHRASP
10   LYSTYRGLYARGGLYLEUALATYRILETYR
11   ALAASPGLYLYSMETVALASNGLUALALEU
12   VALARGGLNGLYLEUALALYSVALALATYR
13   VALTYRLYSPROASNASNTHRHISGLUGLN
14   HISLEUARGLYSSERGLUALAGLNALALYS
15   LYSGLULYSLEUASNILETRPSERGLUASN
16   ASPALAASPSERGLYGLN

Samples:

sample_one:

sample_condition_set_one: pH: 7.4 na; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablesample_condition_set_one

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

BMRB 136 1581 1582 16585 1704 17718 1874 1875 1876 1877 1878 188 189 2784 2785 4010 4052 4053 4905 494 495 496 497 530 5536 6250 6251 6907 6908
PDB
DBJ BAB94634 BAF67032
EMBL CAA24594 CAG39855 CAG42530 CAQ49298 CBI48745
GB AAC14660 AAW36415 ABD22328 ABD29945 ABF58092
PRF 1109959A 710414A
REF NP_645586 WP_000141556 WP_001548082 WP_001566557 WP_001574556
SP P00644 Q5HHM4 Q6GB41 Q6GIK1 Q8NXI6