BMRB Entry 6447
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6447
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Title: Solution structure of a two-repeat fragment of major vault protein PubMed: 16373071
Deposition date: 2004-12-28 Original release date: 2007-01-29
Authors: Kozlov, G.; Vavelyuk, O.; Minailiuc, O.; Banville, D.; Gehring, K.; Ekiel, I.
Citation: Kozlov, G.; Vavelyuk, O.; Minailiuc, O.; Banville, D.; Gehring, K.; Ekiel, I.. "Solution Structure of a Two-repeat Fragment of Major Vault Protein." J. Mol. Biol. 356, 444-452 (2006).
Assembly members:
Major vault protein, polymer, 113 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Major vault protein: GSHMQVVLPNTALHLKALLD
FEDKDGDKVVAGDEWLFEGP
GTYIPRKEVEVVEIIQATII
RQNQALRLRARKECWDRDGK
ERVTGEEWLVTTVGAYLPAV
FEEVLDLVDAVIL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 204 |
| 15N chemical shifts | 110 |
| 1H chemical shifts | 642 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | mv_protein | 1 |
Entities:
Entity 1, mv_protein 113 residues - Formula weight is not available
| 1 | GLY | SER | HIS | MET | GLN | VAL | VAL | LEU | PRO | ASN | ||||
| 2 | THR | ALA | LEU | HIS | LEU | LYS | ALA | LEU | LEU | ASP | ||||
| 3 | PHE | GLU | ASP | LYS | ASP | GLY | ASP | LYS | VAL | VAL | ||||
| 4 | ALA | GLY | ASP | GLU | TRP | LEU | PHE | GLU | GLY | PRO | ||||
| 5 | GLY | THR | TYR | ILE | PRO | ARG | LYS | GLU | VAL | GLU | ||||
| 6 | VAL | VAL | GLU | ILE | ILE | GLN | ALA | THR | ILE | ILE | ||||
| 7 | ARG | GLN | ASN | GLN | ALA | LEU | ARG | LEU | ARG | ALA | ||||
| 8 | ARG | LYS | GLU | CYS | TRP | ASP | ARG | ASP | GLY | LYS | ||||
| 9 | GLU | ARG | VAL | THR | GLY | GLU | GLU | TRP | LEU | VAL | ||||
| 10 | THR | THR | VAL | GLY | ALA | TYR | LEU | PRO | ALA | VAL | ||||
| 11 | PHE | GLU | GLU | VAL | LEU | ASP | LEU | VAL | ASP | ALA | ||||
| 12 | VAL | ILE | LEU |
Samples:
sample_1: Major vault protein, [U-15N], 1 mM; bis-Tris 20 mM; NaCl 150 mM; CaCl2 2 mM; DTT 15 mM; H2O 90%; D2O 10%
sample_cond_1: ionic strength: 150 mM; pH: 6.8; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | not available | not available | not available |
| HNHA | not available | not available | not available |
| 2D NOESY | not available | not available | not available |
Software:
xwinnmr v2.1 - collection, processing
XEASY v1.3.13 - data analysis
CYANA v1.0.6 - structure solution
X-PLOR NIH v2.9.2 - refinement
NMR spectrometers:
- Bruker DRX 500 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts