BMRB Entry 6479

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6479

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: The Structure of the Excisionase (Xis) Protein from Conjugative Transposon Tn916 Provides Insights into the Regulation of Heterobivalent Tyrosine Recombinases PubMed: 15733914

Deposition date: 2005-02-02 Original release date: 2005-04-04

Authors: Abbani, M.; Iwahara, M.; Clubb, R.

Citation: Abbani, M.; Iwahara, M.; Clubb, R.. "The structure of the excisionase (Xis) protein from conjugative transposon Tn916 provides insights into the regulation of heterobivalent tyrosine recombinases " J. Mol. Biol. 374, 11-25 (2005).

Assembly members:
Excisionase from transposon Tn916, polymer, 70 residues, Formula weight is not available

Natural source: Common Name: Enterococcus faecalis Taxonomy ID: 1351 Superkingdom: Eubacteria Kingdom: not available Genus/species: Enterococcus faecalis

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):
Excisionase from transposon Tn916: AGHMKQTDIPIWERYTLTIE EASKYFRIGENKLRRLAEEN KNANWLIMNGNRIQIKRKQF EKIIDTLDAI

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1
coupling_constants
- coupling_constants_set_1

Data type	Count
1H chemical shifts	458
13C chemical shifts	310
15N chemical shifts	77
coupling constants	32

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Excisionase from transposon Tn916	1

Entities:

Entity 1, Excisionase from transposon Tn916 70 residues - Formula weight is not available

1	ALA	GLY	HIS	MET	LYS	GLN	THR	ASP	ILE	PRO
2	ILE	TRP	GLU	ARG	TYR	THR	LEU	THR	ILE	GLU
3	GLU	ALA	SER	LYS	TYR	PHE	ARG	ILE	GLY	GLU
4	ASN	LYS	LEU	ARG	ARG	LEU	ALA	GLU	GLU	ASN
5	LYS	ASN	ALA	ASN	TRP	LEU	ILE	MET	ASN	GLY
6	ASN	ARG	ILE	GLN	ILE	LYS	ARG	LYS	GLN	PHE
7	GLU	LYS	ILE	ILE	ASP	THR	LEU	ASP	ALA	ILE

Samples:

sample_1: Excisionase from transposon Tn916, [U-13C; U-15N], 1 mM; NaOAc 30 mM; NaCl 60 mM; H2O 93%; D2O 7%

sample_cond_1: pH: 4.5 na; temperature: 303 K; ionic strength: 60 mM; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-13C CT-HSQC	not available	not available	not available
2D 1H-15N HSQC	not available	not available	not available
3D 1H-13C-1H HCCH-COSY	not available	not available	not available
3D 1H-1H-15N NOESY	not available	not available	not available
3D 13C-1H-1H NOESY	not available	not available	not available
3D 1H-1H-15N TOCSY	not available	not available	not available
3D 1H-13C-1H HCCH-TOCSY	not available	not available	not available
3D CBCA(CO)NH	not available	not available	not available
3D HNCACB	not available	not available	not available
3D HCACO	not available	not available	not available
3D HNCA	not available	not available	not available
3D HNCO	not available	not available	not available
3D HN(CO)CA	not available	not available	not available
3D HNHA	not available	not available	not available
3D HNHB	not available	not available	not available
4D 1H-15N-1H-13C NOESY	not available	not available	not available

Software:

XWINNMR v2.6 - collection

X-PLOR v3.1 - processing

NMRPipe vsgi6x - processing

Pipp v4.3.5 - data analysis

XPLOR-NIH - refinement

NMR spectrometers:

Bruker AMX 500 MHz
Bruker AMX 600 MHz

PDB	1Y6U
DBJ	BAG12509 BAG80636 BAK28445 GAD37681
EMBL	CAA43359 CAO82957 CAP45545 CAQ49381 CAR69111
GB	AAA19426 AAB60029 AAC36981 AAM99802 AAY63949
PRF	2114402X
REF	NP_687930 WP_000814511 WP_000814512 WP_006417979 WP_006730246
SP	P61623 P61624

Biological Magnetic Resonance Data Bank