BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6513

Title: NMR Structure of the nonstructural Protein 7 (nsP7) from the SARS Corona Virus   PubMed: 16188992

Deposition date: 2005-02-18 Original release date: 2007-03-19

Authors: Peti, Wolfgang; Herrmann, Torsten; Johnson, Maggie; Wuthrich, Kurt

Citation: Peti, Wolfgang; Johnson, Maggie; Herrmann, Torsten; Neuman, B.; Buchmeier, M.; Nelson, M.; Joseph, J.; Page, R.; Stevens, R.; Kuhn, P.; Wuthrich, Kurt. "Structural genomics of the SARS coronavirus: NMR structure of the protein nsp7"  J. Virol. 79, 12905-12913 (2005).

Assembly members:
non structural protein 7, polymer, 85 residues, Formula weight is not available

Natural source:   Common Name: corona virus   Taxonomy ID: not available   Superkingdom: Viruses   Kingdom: not available   Genus/species: SARS corona virus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
non structural protein 7: GHSKMSDVKCTSVVLLSVLQ QLRVESSSKLWAQCVQLHND ILLAKDTTEAFEKMVSLLSV LLSMQGAVDINRLCEEMLDN RATLQ

Data sets:
Data typeCount
1H chemical shifts504
13C chemical shifts323
15N chemical shifts75

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1nsp7, monomer1

Entities:

Entity 1, nsp7, monomer 85 residues - Formula weight is not available

1   GLYHISSERLYSMETSERASPVALLYSCYS
2   THRSERVALVALLEULEUSERVALLEUGLN
3   GLNLEUARGVALGLUSERSERSERLYSLEU
4   TRPALAGLNCYSVALGLNLEUHISASNASP
5   ILELEULEUALALYSASPTHRTHRGLUALA
6   PHEGLULYSMETVALSERLEULEUSERVAL
7   LEULEUSERMETGLNGLYALAVALASPILE
8   ASNARGLEUCYSGLUGLUMETLEUASPASN
9   ARGALATHRLEUGLN

Samples:

sample1: non structural protein 7, [U-15N], 2.3 mM; DTT 5 mM

sample2: non structural protein 7, [U-13C; U-15N], 3.2 mM; DTT 5 mM

sample3: non structural protein 7 1 mM; DTT 5 mM

cond1: pH: 7.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1h15nhsqcnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCACONHnot availablenot availablenot available
15N NOESYnot availablenot availablenot available
13C NOESYnot availablenot availablenot available
HCCH TOCSYnot availablenot availablenot available

Software:

CARA v0.9 -

NMR spectrometers:

  • BRUKER AVANCE 600 MHz
  • BRUKER AVANCE 900 MHz

Related Database Links:

BMRB 16981
PDB
DBJ BAC81346 BAC81347 BAC81360 BAC81361 BAC81374
GB AAP13439 AAP13442 AAP13566 AAP13575 AAP30028
REF NP_828849 NP_828850 NP_828865
SP P0C6F5 P0C6F8 P0C6T7 P0C6U8 P0C6V9

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts