BMRB Entry 6624

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PDB ID: 2bye
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6624
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Backbone 1H, 13C, and 15N Resonance Assignments for the 13kD Ras Association Domain 1 (RA1) from Phospholipase C (epsilon) PubMed: 16258837

Deposition date: 2005-05-11 Original release date: 2005-11-07

Authors: Harris, Richard; Bunney, Tom; Katan, Matilda; Driscoll, Paul

Citation: Harris, Richard; Bunney, Tom; Katan, Matilda; Driscoll, Paul. "Backbone 1H, 13C, and 15N Resonance Assignments for the two 13 kD Ras Associating Domains (RA1 and RA2) from Phospholipase C(epsilon)" J. Biomol. NMR 33, 138-138 (2005).

Assembly members:
ra1, polymer, 110 residues, 12788.6 Da.

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):
ra1: GEERKCLQTHRVTVHGVPGP EPFTVFTINGGTKAKQLLQQ ILTNEQDIKPVTTDYFLMEE KYFISKEKNECRKQPFQRAI GPEEEIMQILSSWFPEEGYM GRIVLKTQQE

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	362
15N chemical shifts	92
1H chemical shifts	674

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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1	GLY	GLU	GLU	ARG	LYS	CYS	LEU	GLN	THR	HIS
2	ARG	VAL	THR	VAL	HIS	GLY	VAL	PRO	GLY	PRO
3	GLU	PRO	PHE	THR	VAL	PHE	THR	ILE	ASN	GLY
4	GLY	THR	LYS	ALA	LYS	GLN	LEU	LEU	GLN	GLN
5	ILE	LEU	THR	ASN	GLU	GLN	ASP	ILE	LYS	PRO
6	VAL	THR	THR	ASP	TYR	PHE	LEU	MET	GLU	GLU
7	LYS	TYR	PHE	ILE	SER	LYS	GLU	LYS	ASN	GLU
8	CYS	ARG	LYS	GLN	PRO	PHE	GLN	ARG	ALA	ILE
9	GLY	PRO	GLU	GLU	GLU	ILE	MET	GLN	ILE	LEU
10	SER	SER	TRP	PHE	PRO	GLU	GLU	GLY	TYR	MET
11	GLY	ARG	ILE	VAL	LEU	LYS	THR	GLN	GLN	GLU

Name	Sample	Sample state	Sample conditions
1H15N HSQC	not available	not available	13C
13C1H HSQC	not available	not available	13C
HNCA	not available	not available	13C
HNcoCA	not available	not available	13C
HNCACB	not available	not available	13C
CBCAcoNH	not available	not available	13C
HNCO	not available	not available	13C
HAcacoNH	not available	not available	13C
HCCH-TOCSY	not available	not available	13C
13C NOESY	not available	not available	13C
15N NOESY	not available	not available	13C

PDB	2BYE
DBJ	BAA96040 BAB14090 BAG10469
GB	AAF22005 AAG17145 AAG28341 AAI40706 AAI44287
REF	NP_001159451 NP_001275918 NP_057425 XP_003825424 XP_003825425
SP	Q9P212

Biological Magnetic Resonance Data Bank

BMRB Entry 6624

Title: Backbone 1H, 13C, and 15N Resonance Assignments for the 13kD Ras Association Domain 1 (RA1) from Phospholipase C (epsilon) PubMed: 16258837

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	GLY	GLU	GLU	ARG	LYS	CYS	LEU	GLN	THR	HIS
2	ARG	VAL	THR	VAL	HIS	GLY	VAL	PRO	GLY	PRO
3	GLU	PRO	PHE	THR	VAL	PHE	THR	ILE	ASN	GLY
4	GLY	THR	LYS	ALA	LYS	GLN	LEU	LEU	GLN	GLN
5	ILE	LEU	THR	ASN	GLU	GLN	ASP	ILE	LYS	PRO
6	VAL	THR	THR	ASP	TYR	PHE	LEU	MET	GLU	GLU
7	LYS	TYR	PHE	ILE	SER	LYS	GLU	LYS	ASN	GLU
8	CYS	ARG	LYS	GLN	PRO	PHE	GLN	ARG	ALA	ILE
9	GLY	PRO	GLU	GLU	GLU	ILE	MET	GLN	ILE	LEU
10	SER	SER	TRP	PHE	PRO	GLU	GLU	GLY	TYR	MET
11	GLY	ARG	ILE	VAL	LEU	LYS	THR	GLN	GLN	GLU

1	GLY	GLU	GLU	ARG	LYS	CYS	LEU	GLN	THR	HIS
2	ARG	VAL	THR	VAL	HIS	GLY	VAL	PRO	GLY	PRO
3	GLU	PRO	PHE	THR	VAL	PHE	THR	ILE	ASN	GLY
4	GLY	THR	LYS	ALA	LYS	GLN	LEU	LEU	GLN	GLN
5	ILE	LEU	THR	ASN	GLU	GLN	ASP	ILE	LYS	PRO
6	VAL	THR	THR	ASP	TYR	PHE	LEU	MET	GLU	GLU
7	LYS	TYR	PHE	ILE	SER	LYS	GLU	LYS	ASN	GLU
8	CYS	ARG	LYS	GLN	PRO	PHE	GLN	ARG	ALA	ILE
9	GLY	PRO	GLU	GLU	GLU	ILE	MET	GLN	ILE	LEU
10	SER	SER	TRP	PHE	PRO	GLU	GLU	GLY	TYR	MET
11	GLY	ARG	ILE	VAL	LEU	LYS	THR	GLN	GLN	GLU

1	GLY	GLU	GLU	ARG	LYS	CYS	LEU	GLN	THR	HIS
2	ARG	VAL	THR	VAL	HIS	GLY	VAL	PRO	GLY	PRO
3	GLU	PRO	PHE	THR	VAL	PHE	THR	ILE	ASN	GLY
4	GLY	THR	LYS	ALA	LYS	GLN	LEU	LEU	GLN	GLN
5	ILE	LEU	THR	ASN	GLU	GLN	ASP	ILE	LYS	PRO
6	VAL	THR	THR	ASP	TYR	PHE	LEU	MET	GLU	GLU
7	LYS	TYR	PHE	ILE	SER	LYS	GLU	LYS	ASN	GLU
8	CYS	ARG	LYS	GLN	PRO	PHE	GLN	ARG	ALA	ILE
9	GLY	PRO	GLU	GLU	GLU	ILE	MET	GLN	ILE	LEU
10	SER	SER	TRP	PHE	PRO	GLU	GLU	GLY	TYR	MET
11	GLY	ARG	ILE	VAL	LEU	LYS	THR	GLN	GLN	GLU