BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6796

Title: NMR assignments of the dimeric S. aureus small multidrug-resistance pump in LPPG micelles   PubMed: 16456704

Deposition date: 2005-08-29 Original release date: 2006-03-10

Authors: Poget, Sebastien; Krueger-Koplin, Ray; Krueger-Koplin, Suzanne; Cahill, Sean; Shekar, S.; Girvin, Mark

Citation: Poget, Sebastien; Krueger-Koplin, Suzanne; Krueger-Koplin, Ray; Cahill, Sean; Shekar, S.; Girvin, Mark. "NMR Assignment of the Dimeric S. aureus Small Multidrug-Resistance Pump in LPPG Micelles"  J. Biomol. NMR 36, 10-10 (2006).

Assembly members:
Staphylococcal multidrug-resistance pump (Smr), polymer, 107 residues, Formula weight is not available

Natural source:   Common Name: S. aureus   Taxonomy ID: 1280   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Staphylococcal multidrug-resistance pump (Smr): MPYIYLIIAISTEVIGSAFL KSSEGFSKFIPSLGTIISFG ICFYFLSKTMQHLPLNITYA TWAGLGLVLTTVVSIIIFKE QINLITIVSIVLIIVGVVSL NIFGTSH

Data sets:
Data typeCount
13C chemical shifts310
15N chemical shifts104
1H chemical shifts104

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Smr unit 11
2Smr unit 21

Entities:

Entity 1, Smr unit 1 107 residues - Formula weight is not available

1   METPROTYRILETYRLEUILEILEALAILE
2   SERTHRGLUVALILEGLYSERALAPHELEU
3   LYSSERSERGLUGLYPHESERLYSPHEILE
4   PROSERLEUGLYTHRILEILESERPHEGLY
5   ILECYSPHETYRPHELEUSERLYSTHRMET
6   GLNHISLEUPROLEUASNILETHRTYRALA
7   THRTRPALAGLYLEUGLYLEUVALLEUTHR
8   THRVALVALSERILEILEILEPHELYSGLU
9   GLNILEASNLEUILETHRILEVALSERILE
10   VALLEUILEILEVALGLYVALVALSERLEU
11   ASNILEPHEGLYTHRSERHIS

Samples:

sample_1: Staphylococcal multidrug-resistance pump (Smr), [U-2H; U-13C; U-15N], 1.5 mM; Lysopalmitouylphosphatidylglycerol 10%; Potassioum phosphate buffer 10 mM; Deuterium oxide 5%

conditions_1: pH*: 6.5; temperature: 320 K

Experiments:

NameSampleSample stateSample conditions
1H15N_TROSYnot availableisotropicnot available
1H15N_NOESY-TROSYnot availableisotropicnot available
1H15N_3D-TROSY-NOESY-TROSYnot availableisotropicnot available
TRHNCOnot availableisotropicnot available
TRHN(CA)COnot availableisotropicnot available
TRHNCAnot availableisotropicnot available
TRHN(CO)CAnot availableisotropicnot available
TRHNCACBnot availableisotropicnot available
TRHN(CO)CACBnot availableisotropicnot available
4D-TRHNCACOnot availableisotropicnot available
4D-TRHNCOCAnot availableisotropicnot available

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 700 MHz
  • Bruker Avance 600 MHz
  • Bruker Avance 500 MHz
  • Bruker Avance 900 MHz

Related Database Links:

BMRB 16783
DBJ BAD01019 BAE92298 BAP94573
EMBL CAA33598 CAA44471 CAA44472 CAA86016 CAC14916
GB AAA26666 AAA26668 AAA60379 AAB02114 AAB07747
PRF 1907159A
REF NP_647561 NP_863640 NP_878005 NP_940784 WP_001146389
SP P14319 Q55339

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