BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6912

Title: 1H, 13C, and 15N Resonance Assignments for the reduced form of Thioredoxin 1 from Sacharomyces cerevisae   PubMed: 16609834

Deposition date: 2005-12-01 Original release date: 2006-04-14

Authors: Pinheiro, Anderson; Amorim, Gisele; Luis Eduardo, Netto; Ana Paula, Valente; Fabio, Almeida

Citation: Pinheiro, Anderson; Amorim, Gisele; Netto, Luis Eduardo; Valente, Ana Paula; Almeida, Fabio. "1H, 13C and 15N Resonance Assignments for the Reduced Forms of Thioredoxin 1 and 2 from S. cerevisiae"  J. Biomol. NMR 36, 35-35 (2006).

Assembly members:
Trx1, polymer, 103 residues, Formula weight is not available

Natural source:   Common Name: Baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Metazoa

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Trx1: MVTQFKTASEFDSAIAQDKL VVVDFYATWCGPCKMIAPMI EKFSEQYPQADFYKLDVDEL GDVAQKNEVSAMPTLLLFKN GKEVAKVVGANPAAIKQAIA ANA

Data sets:
Data typeCount
13C chemical shifts427
15N chemical shifts105
1H chemical shifts696

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1yeast thioredoxin 11

Entities:

Entity 1, yeast thioredoxin 1 103 residues - Formula weight is not available

1   METVALTHRGLNPHELYSTHRALASERGLU
2   PHEASPSERALAILEALAGLNASPLYSLEU
3   VALVALVALASPPHETYRALATHRTRPCYS
4   GLYPROCYSLYSMETILEALAPROMETILE
5   GLULYSPHESERGLUGLNTYRPROGLNALA
6   ASPPHETYRLYSLEUASPVALASPGLULEU
7   GLYASPVALALAGLNLYSASNGLUVALSER
8   ALAMETPROTHRLEULEULEUPHELYSASN
9   GLYLYSGLUVALALALYSVALVALGLYALA
10   ASNPROALAALAILELYSGLNALAILEALA
11   ALAASNALA

Samples:

sample_1: thioredoxin 2, [U-13C; U-15N], 1 mM; 2-mercaptoethanol 3 mM; H2O 90%; D2O 10%

sample_2: thioredoxin 2, [U-15N], 1.2 mM; DTT 3 mM; H2O 90%; D2O 10%

sample_3: thioredoxin 2 1.2 mM; DTT 3 mM; D2O 99%

conditions_1: pH: 7.0; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availableconditions_1
HN(CO)CAnot availablenot availableconditions_1
HNCACBnot availablenot availableconditions_1
CBCA(CO)NHnot availablenot availableconditions_1
HNCOnot availablenot availableconditions_1
H(CCO)NH-TOCSYnot availablenot availableconditions_1
(H)CC(CO)NH-TOCSYnot availablenot availableconditions_1
HCCH-TOCSYnot availablenot availableconditions_1
1H-1H_TOCSY_2Dnot availablenot availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ GAA24928
EMBL CAA97572 CAY81281
GB AAA35171 AAA35177 AAS56529 AHY78447 EDN59589
REF NP_013144
SP P22217
TPG DAA09361

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
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