BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 7001

Title: Structure of the Phosphorylation Motif of the oncogenic Protein beta-Catenin Recognized By a Selective Monoclonal Antibody   PubMed: 16996060

Deposition date: 2006-02-22 Original release date: 2006-10-19

Authors: Megy, Simon; Bertho, Gildas; Gharbi-Benarous, Josyane; Baleux, Francoise; Benarous, Richard; Girault, Jean-Pierre

Citation: Megy, Simon; Bertho, Gildas; Gharbi-Benarous, Josyane; Baleux, Francoise; Benarous, Richard; Girault, Jean-Pierre. "STD and TRNOESY NMR Studies for the Epitope Mapping of the Phosphorylation Motif of the oncogenic Protein beta-Catenin Recognized By a Selective Monoclonal Antibody"  FEBS Lett. 580, 5411-5422 (2006).

Assembly members:
P-beta-Cat peptide, polymer, 28 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
P-beta-Cat peptide: XKAAVSHWQQQSYLDXGIHX GATTTAPX

Data sets:
Data typeCount
1H chemical shifts171

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1P-beta-Cat 19-441

Entities:

Entity 1, P-beta-Cat 19-44 28 residues - Formula weight is not available

Two phosphorylated serines (SEP) are present in the sequence at positions 15 and 19: DpSIHpS

1   ACELYSALAALAVALSERHISTRPGLNGLN
2   GLNSERTYRLEUASPSEPGLYILEHISSEP
3   GLYALATHRTHRTHRALAPRONH2

Samples:

sample_1: P-beta-Cat peptide, not labeled, 2 ± 0.1 mM; Phosphate buffer mM

conditions_1: pH: 7.2; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
NOESYsample_1not availableconditions_1

Software:

X-WIN-NMR v3.5, Biospin Bruker - processing

NMR spectrometers:

  • Biospin Bruker Avance 500 MHz

Related Database Links:

PDB