BMRB Entry 7035
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7035
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for 2nd SH3 domain of human NCK2 adaptor protein PubMed: 16752908
Deposition date: 2006-03-29 Original release date: 2007-09-24
Authors: Liu, Jingxian
Citation: Liu, Jingxian; Li, Minfen; Ran, Xiaoyuan; Fan, Jing-song; Song, Jianxing. "Structural Insight into the Binding Diversity between the Human Nck2 SH3 Domains and Proline-Rich Proteins" Biochemistry 45, 7171-7184 (2006).
Assembly members:
NCK2 2nd SH3, polymer, 57 residues, 6559 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
NCK2 2nd SH3: IPAFVKFAYVAEREDELSLV
KGSRVTVMEKCSDGWWRGSY
NGQIGWFPSNYVLEEVD
- assigned_chemical_shifts
- heteronucl_T1_relaxation
- heteronucl_T2_relaxation
Data type | Count |
13C chemical shifts | 99 |
15N chemical shifts | 53 |
1H chemical shifts | 311 |
T1 relaxation values | 63 |
T2 relaxation values | 63 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | NCK2 2nd SH3 | 1 |
Entities:
Entity 1, NCK2 2nd SH3 57 residues - 6559 Da.
1 | ILE | PRO | ALA | PHE | VAL | LYS | PHE | ALA | TYR | VAL | ||||
2 | ALA | GLU | ARG | GLU | ASP | GLU | LEU | SER | LEU | VAL | ||||
3 | LYS | GLY | SER | ARG | VAL | THR | VAL | MET | GLU | LYS | ||||
4 | CYS | SER | ASP | GLY | TRP | TRP | ARG | GLY | SER | TYR | ||||
5 | ASN | GLY | GLN | ILE | GLY | TRP | PHE | PRO | SER | ASN | ||||
6 | TYR | VAL | LEU | GLU | GLU | VAL | ASP |
Samples:
sample_1: NCK2 2nd SH3, [U-95% 15N], 1.4 ± 0.1 mM; phosphate 20 ± 2 mM; DTT 10 ± 2 mM; D2O 10 ± 2 %
sample_2: NCK2 2nd SH3, [U-95% 13C; U-95% 15N], 1.4 ± 0.1 mM; phosphate 20 ± 2 mM; DTT 10 ± 2 mM; D2O 70 ± 2 %
sample_3: NCK2 2nd SH3, [U-95% 13C; U-95% 15N], 1.4 ± 0.1 mM; phosphate 20 ± 2 mM; DTT 10 ± 2 mM; D2O 10 ± 2 %
conditions_1: pH: 6.8; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
1H15N HSQC | not available | not available | conditions_1 |
HSQC-TOCSY | sample_1 | not available | conditions_1 |
HSQC-NOESY | sample_1 | not available | conditions_1 |
HNCACB/CBCANH | sample_3 | not available | conditions_1 |
HCCH TOCSY/HCCH NOESY | not available | not available | conditions_1 |
T1/T2/HetNOE | not available | not available | conditions_1 |
Software:
NMRPipe - raw data processing
NMRView - peak assignment
NMR spectrometers:
- Bruker Avance 800 MHz
Related Database Links:
PDB | |
DBJ | BAI47129 |
GB | AAC04831 AAC80284 AAH00103 AAH07195 AAY24332 |
REF | NP_001004720 NP_003572 XP_001109452 XP_001109500 XP_001488099 |
SP | O43639 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts