BMRB Entry 7061
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR7061
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Title: NMR structure of talin-PTB in complex with PIPKI PubMed: 16616931
Deposition date: 2006-04-10 Original release date: 2008-07-17
Authors: Kong, X.; Wang, X.; Misra, S.; Qin, J.
Citation: Kong, X.; Wang, X.; Misra, S.; Qin, J.. "Structural Basis for the Phosphorylation-regulated Focal Adhesion Targeting of Type Igamma Phosphatidylinositol Phosphate Kinase (PIPKIgamma) by Talin" J. Mol. Biol. 359, 47-54 (2006).
Assembly members:
Talin-1, polymer, 106 residues, Formula weight is not available
SWVXSPLH, polymer, 8 residues, 261.168 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Talin-1: LKTYGVSFFLVKEKMKGKNK
LVPRLLGITKECVMRVDEKT
KEVIQEWSLTNIKRWAASPK
SFTLDFGDYQDGYYSVQTTE
GEQIAQLIAGYIDIILKKKK
SKDHFG
SWVXSPLH: SWVXSPLH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 441 |
| 15N chemical shifts | 123 |
| 1H chemical shifts | 740 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Talin-1 | 1 |
| 2 | peptide | 2 |
Entities:
Entity 1, Talin-1 106 residues - Formula weight is not available
| 1 | LEU | LYS | THR | TYR | GLY | VAL | SER | PHE | PHE | LEU | ||||
| 2 | VAL | LYS | GLU | LYS | MET | LYS | GLY | LYS | ASN | LYS | ||||
| 3 | LEU | VAL | PRO | ARG | LEU | LEU | GLY | ILE | THR | LYS | ||||
| 4 | GLU | CYS | VAL | MET | ARG | VAL | ASP | GLU | LYS | THR | ||||
| 5 | LYS | GLU | VAL | ILE | GLN | GLU | TRP | SER | LEU | THR | ||||
| 6 | ASN | ILE | LYS | ARG | TRP | ALA | ALA | SER | PRO | LYS | ||||
| 7 | SER | PHE | THR | LEU | ASP | PHE | GLY | ASP | TYR | GLN | ||||
| 8 | ASP | GLY | TYR | TYR | SER | VAL | GLN | THR | THR | GLU | ||||
| 9 | GLY | GLU | GLN | ILE | ALA | GLN | LEU | ILE | ALA | GLY | ||||
| 10 | TYR | ILE | ASP | ILE | ILE | LEU | LYS | LYS | LYS | LYS | ||||
| 11 | SER | LYS | ASP | HIS | PHE | GLY |
Entity 2, peptide 8 residues - 261.168 Da.
| 1 | SER | TRP | VAL | PTR | SER | PRO | LEU | HIS |
Samples:
sample_1: Talin-1, [U-13C; U-15N], 1 mM; SWVXSPLH 1 mM; phosphate buffer 20 mM; Ca2+ 5 mM; D2O 10%; H2O 90%
sample_cond_1: pH: 6.3; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCACB | sample_1 | not available | sample_cond_1 |
| CBCA(CO)NH | sample_1 | not available | sample_cond_1 |
| HCCH-TOCSY | sample_1 | not available | sample_cond_1 |
| HNCO | sample_1 | not available | sample_cond_1 |
| edited NOESY | sample_1 | not available | sample_cond_1 |
| filtered NOESY | sample_1 | not available | sample_cond_1 |
Software:
TALOS - data analysis
X-PLOR - refinement
NMR spectrometers:
- VARIAN INOVA 600 MHz
Related Database Links:
| BMRB | 15792 |
| PDB | |
| DBJ | BAA82979 BAC30516 BAC65702 BAE27781 BAG09941 |
| EMBL | CAA39588 CAI10978 CAM17190 |
| GB | AAD13152 AAF23322 AAF27330 AAH42923 AAI21760 |
| PRF | 1617167A |
| REF | NP_001034114 NP_006280 NP_035732 NP_989854 XP_001084941 |
| SP | P26039 P54939 Q9Y490 |
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