BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7068

Title: Solution structure and intermolecular interactions of the Copper form of third metal-binding domain of ATP7A, the menkes disease protein   PubMed: 16873374

Deposition date: 2006-04-10 Original release date: 2008-07-07

Authors: Banci, L.; Bertini, I.; Cantini, F.; DellaMalva, N.; Rosato, A.; Herrmann, T.; Wuthrich, K.

Citation: Banci, L.; Bertini, I.; Cantini, F.; DellaMalva, N.; Herrmann, T.; Rosato, A.; Wuthrich, K.. "Solution structure and intermolecular interactions of the third metal-binding domain of ATP7A, the Menkes disease protein"  J. Biol. Chem. 281, 29141-29147 (2006).

Assembly members:
Copper-transporting ATPase 1, polymer, 90 residues, Formula weight is not available
CU1, non-polymer, 63.546 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Copper-transporting ATPase 1: NDSTATFIIDGMHCKSCVSN IESTLSALQYVSSIVVSLEN RSAIVVYNASSVTPESLRKA IEAVSPGLYRVSITSEVEIE GRLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts243
15N chemical shifts78
1H chemical shifts536

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Copper-transporting ATPase 11
2COPPER (I) ION2

Entities:

Entity 1, Copper-transporting ATPase 1 90 residues - Formula weight is not available

1   ASNASPSERTHRALATHRPHEILEILEASP
2   GLYMETHISCYSLYSSERCYSVALSERASN
3   ILEGLUSERTHRLEUSERALALEUGLNTYR
4   VALSERSERILEVALVALSERLEUGLUASN
5   ARGSERALAILEVALVALTYRASNALASER
6   SERVALTHRPROGLUSERLEUARGLYSALA
7   ILEGLUALAVALSERPROGLYLEUTYRARG
8   VALSERILETHRSERGLUVALGLUILEGLU
9   GLYARGLEUGLUHISHISHISHISHISHIS

Entity 2, COPPER (I) ION - Cu - 63.546 Da.

1   CU1

Samples:

sample_1: Copper-transporting ATPase 1 1 mM; phosphate_buffer 100 mM; H2O 90%; D2O 10%

sample_2: Copper-transporting ATPase 1, [U-15N], 0.5 mM; phosphate_buffer 100 mM; H2O 90%; D2O 10%

sample_3: Copper-transporting ATPase 1, [U-15N; U-13C], 0.5 mM; phosphate_buffer 100 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 100 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablesample_cond_1
2D TOCSYnot availablenot availablesample_cond_1
3D 15N-separated NOESYnot availablenot availablesample_cond_1
CBCACONHnot availablenot availablesample_cond_1
CBCANHnot availablenot availablesample_cond_1
h(CCH)-TOCSYnot availablenot availablesample_cond_1
3D 13C-separated NOESYnot availablenot availablesample_cond_1
15N-HSQCnot availablenot availablesample_cond_1

Software:

xwinnmr v1.3 - collection

XEASY v1.3 - data analysis

CARA v1.5 - data analysis

CYANA v2.1 - structure solution

AMBER v8.0 - refinement

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
BMRB 7069

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts