BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7089

Title: 1H, 13C, and 15N Peak Assignments of Human Macrophage Metalloelastase, in its inhibitor-free state   PubMed: 16855860

Deposition date: 2006-04-26 Original release date: 2007-05-02

Authors: Bhaskaran, Rajagopalan; VanDoren, Steven

Citation: Bhaskaran, Rajagopalan; VanDoren, Steven. "1H, 13C, and 15N peak assignments and secondary structure of human macrophage metalloelastase (MMP-12) in its inhibitor-free state"  J. Biomol. NMR 36, 55-55 (2006).

Assembly members:
MMP-12, polymer, 164 residues, 18152.7 Da.
ZN, non-polymer, 65.409 Da.
CA, non-polymer, 40.078 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
MMP-12: FREMPGGPVWRKHYITYRIN NYTPDMNREDVDYAIRKAFQ VWSNVTPLKFSKINTGMADI LVVFARGAHGDFHAFDGKGG ILAHAFGPGSGIGGDAHFDE DEFWTTHSGGTNLFLTAVHA IGHSLGLGHSSDPKAVMFPT YKYVDINTFRLSADDIRGIQ SLYG

Data sets:
Data typeCount
13C chemical shifts638
15N chemical shifts166
1H chemical shifts958

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Metalloprotease1
2ZINC (II) ION, 12
3ZINC (II) ION, 22
4CALCIUM (II) ION, 13
5CALCIUM (II) ION, 23
6CALCIUM (II) ION, 33

Entities:

Entity 1, Metalloprotease 164 residues - 18152.7 Da.

The residue's author sequnce code corresponds to the original publication of the full-length protein sequence

1   PHEARGGLUMETPROGLYGLYPROVALTRP
2   ARGLYSHISTYRILETHRTYRARGILEASN
3   ASNTYRTHRPROASPMETASNARGGLUASP
4   VALASPTYRALAILEARGLYSALAPHEGLN
5   VALTRPSERASNVALTHRPROLEULYSPHE
6   SERLYSILEASNTHRGLYMETALAASPILE
7   LEUVALVALPHEALAARGGLYALAHISGLY
8   ASPPHEHISALAPHEASPGLYLYSGLYGLY
9   ILELEUALAHISALAPHEGLYPROGLYSER
10   GLYILEGLYGLYASPALAHISPHEASPGLU
11   ASPGLUPHETRPTHRTHRHISSERGLYGLY
12   THRASNLEUPHELEUTHRALAVALHISALA
13   ILEGLYHISSERLEUGLYLEUGLYHISSER
14   SERASPPROLYSALAVALMETPHEPROTHR
15   TYRLYSTYRVALASPILEASNTHRPHEARG
16   LEUSERALAASPASPILEARGGLYILEGLN
17   SERLEUTYRGLY

Entity 2, ZINC (II) ION, 1 - Zn - 65.409 Da.

1   ZN

Entity 3, CALCIUM (II) ION, 1 - Ca - 40.078 Da.

1   CA

Samples:

sample_1: MMP-12, [U-13C; U-15N], 0.55 mM

sample_2: MMP-12, [U-15N], 0.45 mM

conditions_1: pH: 6.6; temperature: 299 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCnot availablenot availableconditions_1
1H13C HSQCnot availablenot availableconditions_1
HNCOnot availablenot availableconditions_1
HNCAnot availablenot availableconditions_1
CBCACONHnot availablenot availableconditions_1
HNCACBnot availablenot availableconditions_1
HACACONHnot availablenot availableconditions_1
HNNCAHAnot availablenot availableconditions_1
CCONHnot availablenot availableconditions_1
HCCONHnot availablenot availableconditions_1
HCACOCANHnot availablenot availableconditions_1
HCCHTOCSYnot availablenot availableconditions_1
CCHTOCSYnot availablenot availableconditions_1
HBCBCGCDHD(CEHE)not availablenot availableconditions_1
15N ed 3D NOESY HSQCnot availablenot availableconditions_1
13C ed 3D NOESY HSQCnot availablenot availableconditions_1

Software:

NMRPipe v2.3 - Processing Spectra

SPARKY v3.106 - Assignment, Peak Picking

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Bruker DRX 500 MHz

Related Database Links:

BMRB 15578 6391 6444 7415
PDB
DBJ BAG36675 BAJ20684
GB AAA58658 AAI12302 AAI43774 AAW29944 ADR83017
REF NP_002417 XP_003828422 XP_004052087 XP_508724
SP P39900

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts