BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 7204

Title: Resonance assignments of a CoA binding protein from Klebsiella pneumoniae   PubMed: 17031526

Deposition date: 2006-07-03 Original release date: 2006-10-02

Authors: Hung, Kuo-Wei; Fan, Pei-Ju; Lin, Yi-Chao; Cheng, Chun-Chia; Chang, Chi-Fon; Tsai, Shih-Feng; Huang, Tai-Huang

Citation: Hung, Kuo-Wei; Lin, Yi-Chao; Cheng, Chun-Chia; Fan, Pei-Ju; Chang, Chi-Fon; Tsai, Shih-Feng; Huang, Tai-Huang. "Resonance assignments of a CoA binding protein from Klebsiella pneumoniae"  J. Biomol. NMR 36, 74-74 (2006).

Assembly members:
KP1966, polymer, 145 residues, 15781.2 Da.

Natural source:   Common Name: Klebsiella pneumoniae   Taxonomy ID: 573   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Klebsiella pneumoniae

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
KP1966: MKENDIAGILTSTRTIALVG ASDKPDRPSYRVMKYLLDQG YHVIPVSPKVAGKTLLGQQG YATLADVPEKVDMVDVFRNS EAAWGVAQEAIAIGAKTLWL QLGVINEQAAVLAREAGLSV VMDRCPAIELPRLGLAKLEH HHHHH

Data sets:
Data typeCount
13C chemical shifts587
15N chemical shifts142
1H chemical shifts944

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1KP1966 monomer1

Entities:

Entity 1, KP1966 monomer 145 residues - 15781.2 Da.

1   METLYSGLUASNASPILEALAGLYILELEU
2   THRSERTHRARGTHRILEALALEUVALGLY
3   ALASERASPLYSPROASPARGPROSERTYR
4   ARGVALMETLYSTYRLEULEUASPGLNGLY
5   TYRHISVALILEPROVALSERPROLYSVAL
6   ALAGLYLYSTHRLEULEUGLYGLNGLNGLY
7   TYRALATHRLEUALAASPVALPROGLULYS
8   VALASPMETVALASPVALPHEARGASNSER
9   GLUALAALATRPGLYVALALAGLNGLUALA
10   ILEALAILEGLYALALYSTHRLEUTRPLEU
11   GLNLEUGLYVALILEASNGLUGLNALAALA
12   VALLEUALAARGGLUALAGLYLEUSERVAL
13   VALMETASPARGCYSPROALAILEGLULEU
14   PROARGLEUGLYLEUALALYSLEUGLUHIS
15   HISHISHISHISHIS

Samples:

sample_1: KP1966, [U-15N; U-13C], 1 mM; Tris-HCl 20 mM; NaCl 50 mM; Glu 50 mM; Arg 50 mM

conditions_1: pH: 7.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCAsample_1not availableconditions_1
HN(CO)CAsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
CBCA(CO)NHsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
HN(CA)COsample_1not availableconditions_1
HBHA(CO)NHsample_1not availableconditions_1
15N-edited NOESY-HSQCsample_1not availableconditions_1
13C-edited NOESY-HSQCsample_1not availableconditions_1
HCCH-TOCSYsample_1not availableconditions_1

Software:

xwinnmr v3.5 -

SPARKY v3.112 -

NMR spectrometers:

  • Bruker AVANCE DRX 600 MHz
  • Bruker AVANCE 500 MHz

Related Database Links:

PDB
DBJ BAH62687
EMBL CCI75907 CCM81564 CCM88670 CCM94842 CCN29098
GB ABR76429 ACI07817 ADC59272 AEJ97521 AEW60570
REF WP_002898434 WP_004144085 WP_004201441 WP_004893145 WP_009308306

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts