BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7228

Title: Solution NMR structure of UPF0107 protein AF_0055, Northeast Structural Genomics Consortium Target GR101 (CASP Target)

Deposition date: 2006-07-14 Original release date: 2010-03-04

Authors: Liu, G.; Atreya, H.; Xu, D.; Sukumaran, D.; Chen, C.; Janjua, H.; Cunningham, K.; Ma, L.-C.; Xiao, R.; Liu, J.; Baran, M.; Swapna, G.; Acton, T.; Rost, B.; Montelione, G.; Szyperski, T.

Citation: Liu, G.; Atreya, H.; Xu, D.; Sukumaran, D.; Chen, C.; Janjua, H.; Cunningham, K.; Ma, L.-C.; Xiao, R.; Liu, J.; Baran, M.; Swapna, G.; Acton, T.; Rost, B.; Montelione, G.; Szyperski, T.. "Solution NMR structure of UPF0107 protein AF_0055, Northeast Structural Genomics Consortium Target GR101 (CASP Target)"  . ., .-..

Assembly members:
UPF0107 protein AF0055, polymer, 141 residues, Formula weight is not available

Natural source:   Common Name: Archaeoglobus fulgidus   Taxonomy ID: 2234   Superkingdom: Archaea   Kingdom: not available   Genus/species: Archaeoglobus fulgidus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
UPF0107 protein AF0055: MVKFACRAITRGRAEGEALV TKEYISFLGGIDKETGIVKE DCEIKGESVAGRILVFPGGK GSTVGSYVLLNLRKNGVAPK AIINKKTETIIAVGAAMAEI PLVEVRDEKFFEAVKTGDRV VVNADEGYVELIELEHHHHH H

Data sets:
Data typeCount
13C chemical shifts454
15N chemical shifts140
1H chemical shifts979

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1UPF0107 protein AF00551

Entities:

Entity 1, UPF0107 protein AF0055 141 residues - Formula weight is not available

1   METVALLYSPHEALACYSARGALAILETHR
2   ARGGLYARGALAGLUGLYGLUALALEUVAL
3   THRLYSGLUTYRILESERPHELEUGLYGLY
4   ILEASPLYSGLUTHRGLYILEVALLYSGLU
5   ASPCYSGLUILELYSGLYGLUSERVALALA
6   GLYARGILELEUVALPHEPROGLYGLYLYS
7   GLYSERTHRVALGLYSERTYRVALLEULEU
8   ASNLEUARGLYSASNGLYVALALAPROLYS
9   ALAILEILEASNLYSLYSTHRGLUTHRILE
10   ILEALAVALGLYALAALAMETALAGLUILE
11   PROLEUVALGLUVALARGASPGLULYSPHE
12   PHEGLUALAVALLYSTHRGLYASPARGVAL
13   VALVALASNALAASPGLUGLYTYRVALGLU
14   LEUILEGLULEUGLUHISHISHISHISHIS
15   HIS

Samples:

sample_1: UPF0107 protein AF0055, [U-15N; U-13C], 1.2 mM; CaCl25 – 5.5 mM; NaCl 100 mM; NH4OAc 20 mM; DTT 10 mM; NaN3 0.02%; D2O 5%; H2O 95%

sample_cond_1: pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
SIMULTANEOUS HETERONUCLEAR RESOLVED [1H,1H]-NOESYsample_1not availablesample_cond_1
GFT (4,3)D HNNCABCAsample_1not availablesample_cond_1
GFT (4,3)D CABCA(CO)NHNsample_1not availablesample_cond_1
GFT (4,3)D HABCAB(CO)NHNsample_1not availablesample_cond_1
GFT (4,3)D HCCHsample_1not availablesample_cond_1

Software:

VNMR v6.1c - collection

NMRPipe v2.3 - processing

AutoAssign v1.15.1 - data analysis

AutoStruct v2.0 - data analysis

CYANA v2.1 - structure solution

CNS v1.1 - refinement

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB AAB91166 AIG96894
REF NP_068896 WP_010877569
SP O30181

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts