BMRB Entry 7245
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR7245
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Title: Structural and Functional Characterization of TM VII of the NHE1 Isoform of the Na+/H+ Exchanger PubMed: 16861220
Deposition date: 2006-07-25 Original release date: 2006-08-12
Authors: Rainey, Jan; Ding, Jie; Xu, Carolyn; Fliegel, Larry; Sykes, Brian
Citation: Ding, Jie; Rainey, Jan; Xu, Carolyn; Sykes, Brian; Fliegel, Larry. "Structural and functional characterization of transmembrane segment VII of the Na+/H+ exchanger isoform 1." J. Biol. Chem. 281, 29817-29829 (2006).
Assembly members:
TM VII of Na+/H+ exchanger, polymer, 28 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
TM VII of Na+/H+ exchanger: HINELLHILVFGESLLNDAV
TVVLYKKX
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 69 |
| 15N chemical shifts | 6 |
| 1H chemical shifts | 226 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TM VII of Na+/H+ exchanger | 1 |
Entities:
Entity 1, TM VII of Na+/H+ exchanger 28 residues - Formula weight is not available
| 1 | HIS | ILE | ASN | GLU | LEU | LEU | HIS | ILE | LEU | VAL | ||||
| 2 | PHE | GLY | GLU | SER | LEU | LEU | ASN | ASP | ALA | VAL | ||||
| 3 | THR | VAL | VAL | LEU | TYR | LYS | LYS | NH2 |
Samples:
sample_1: TM VII, none, 1 mM; DPC, [U-2H], 75 mM; DSS 1 mM
sample_2: TM VII, [15N]-L5,L9,G12,L15,A19,L24, 1 mM; DPC, [U-2H], 75 mM; DSS 1 mM
conditions_1: pH: 4.8; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HSQC (natural abundance) | not available | not available | conditions_1 |
| NOESY | not available | not available | conditions_1 |
| TOCSY | not available | not available | conditions_1 |
| 15N-1H HSQC | not available | not available | conditions_1 |
| 15N-edited NOESY-HSQC (at 500 MHz) | not available | not available | conditions_1 |
| 15N-edited TOCSY-HSQC (at 500 MHz) | not available | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Varian INOVA 800 MHz
- Varian INOVA 500 MHz
Related Database Links:
| BMRB | 19484 |
| PDB | |
| DBJ | BAE02258 BAE29332 BAE32882 BAE38221 BAE75800 |
| EMBL | CAA42558 CAA43721 CAA48771 CAA69925 CAB45232 |
| GB | AAA31092 AAA91483 AAA92976 AAA98479 AAB20633 |
| REF | NP_001007104 NP_001038108 NP_001081553 NP_001095191 NP_001106952 |
| SP | P19634 P23791 P26431 P48761 P48762 |
| TPG | DAA32066 |
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